CD42b antibody

Catalog No. ABIN120669

The Mouse Monoclonal anti-CD42b antibody (Clone HIP1) (ABIN120669) specifically detects CD42b in FACS, IHC (fro) and Func. The antibody is reactive with Human and Primate samples.

Quick Overview for CD42b antibody (ABIN120669)

Target: CD42b (GP1BA) (Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA))

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD42b antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Functional Studies (Func)

Clone: HIP1

Product Details anti-CD42b Antibody

Purification: Protein A Chromatography

Immunogen: Peripheral blood mononuclear cells of a patient suffering with CLL

Isotype: IgG1

Application Details

Application Notes: Flow Cytometry. Immunohistochemistry on frozen sections. Functional Application: The antibody inhibits the ristocetin-dependent binding of von Willebrand Factor (vWF) to platelets and ristocetin-induced platelet agglutination.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM Sodium Azide as preservative, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for CD42b

Target: CD42b (GP1BA) (Glycoprotein Ib (Platelet), alpha Polypeptide (GP1BA))

Alternative Name: CD42b / GPIbA

Background: CD42b (GPIba) composes together with GPIbb, GPIX and GPV the GPIb-IX-V receptor complex critical in the process of platelet-rich thrombus formation by tethering the platelet to a thrombogenic surface. CD42b binds to von Willebrand factor (VWF) exposed at a site of vascular injury, as well as to thrombin, coagulation factors XI and XII, high molecular wight kininogen, TSP-1, integrin Mac-1 and P-selectin. The extracellular domain of CD42b by its interactions also contributes to metastasis.Synonyms: GP-Ib alpha, GPIb-alpha, Glycoprotein Ibalpha, Platelet glycoprotein Ib alpha chain

UniProt: P07359