Blood Group Lewis B antibody

Catalog No. ABIN120686

The Mouse Monoclonal anti-Blood Group Lewis B antibody has been validated for IHC (p), WB and EIA. It is suitable to detect Blood Group Lewis B in samples from Human.

Quick Overview for Blood Group Lewis B antibody (ABIN120686)

Target: Blood Group Lewis B

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Blood Group Lewis B antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 2-25LE

Product Details anti-Blood Group Lewis B Antibody

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity Chromatography on Protein A.

Immunogen: Mucin isolated by size exclusion chromatography from an ovarian cyst fluid (pure endocervical type according to the Fenoglio's classification)

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry: 2-4 μg/mL for 30 min at RT. Staining of formalin-fixed tissues REQUIRES boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min. followed by cooling at RT for 20 min. Use human colon CA for postive control. Western blot. ELISA.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4, with 15 mM Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for 6 months. For long-term storage aliquot and store at -20 °C. Avoid freeze/thaw cycles.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for Blood Group Lewis B

Target: Blood Group Lewis B

Background: Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.Synonyms: Blood Group Antigen Lewis b