ACRV1 antibody

Catalog No. ABIN120713

The Mouse Monoclonal anti-ACRV1 antibody has been validated for WB, IF and FACS. It is suitable to detect ACRV1 in samples from Human and Pig.

Quick Overview for ACRV1 antibody (ABIN120713)

Target: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Reactivity: Human, Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This ACRV1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: Hs-8

Product Details anti-ACRV1 Antibody

Specificity: The antibody reacts with intra-acrosomal sperm proteins (220, 50, 34 and 21 kDa). Reacts with Human, Porcine (Pig).

Characteristics: Synonyms: Acrosomal protein SP-10, SP10, SPACA2, Acrosomal vesicle protein 1, Intra-acrosomalSperm Protein

Purification: Precipitation methods and size-exclusion chromatography

Purity: > 95 % (by SDS-PAGE)

Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid,10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0. 2% aceticacid and subsequently used for immunization.

Isotype: IgM

Application Details

Application Notes: Immunocytochemistry: 10 μg/mL, membrane permeabilization (acetone) essential. Flow Cytometry. Western Blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for ACRV1

Target: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Alternative Name: ACRV1

Background: One of the most frequent causes of man infertility is defective sperm acrosome. This damage can be detected using antibodies against intra-acrosomal proteins. Besides diagnostics of sperm pathology, monoclonal antibodies against intra-acrosomal proteins can be used for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.Synonyms: Acrosomal protein SP-10, Acrosomal vesicle protein 1, Intra-acrosomal Sperm Protein, SP10, SPACA2

Gene ID: 56

UniProt: P26436