ACRV1 antibody (N-Term)

Catalog No. ABIN2785464

Product Details anti-ACRV1 Antibody

Target: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Binding Specificity: N-Term

Reactivity: Human, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACRV1 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: MNRFLLLMSL YLLGSARGTS SQPNELSGSI DHQTSVQQLP GEFFSLENPS

Predicted Reactivity: Guinea Pig: 79%, Human: 100%, Rabbit: 85%

Characteristics: This is a rabbit polyclonal antibody against ACRV1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ACRV1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Comment:

Antigen size: 265 AA

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-ACRV1 antibody (ABIN2785464)

Reddi, Shore, Acharya, Herr: "Transcriptional regulation of spermiogenesis: insights from the study of the gene encoding the acrosomal protein SP-10." in: Journal of reproductive immunology, Vol. 53, Issue 1-2, pp. 25-36, (2001) (PubMed).

Target Details for ACRV1

Target: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Alternative Name: ACRV1 (ACRV1 Products)

Synonyms: ACRV1 antibody, D11S4365 antibody, SP-10 antibody, SPACA2 antibody, Msa63 antibody, Sp10 antibody, acrosomal vesicle protein 1 antibody, ACRV1 antibody, Acrv1 antibody

Background: ACRV1 is a testis-specific, differentiation antigen, acrosomal vesicle protein 1, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. The acrosomal vesicle protein 1 may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans. This gene encodes a testis-specific, differentiation antigen, acrosomal vesicle protein 1, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. This gene consists of 4 exons and its alternative splicing generates multiple distinct transcripts, which encode protein isoforms ranging from 81 to 265 amino acids. The longest transcript is the most abundant, comprising 53-72 % of the total acrosomal vesicle protein 1 messages, the second largest transcript comprises 15-32 %, the third and the fourth largest transcripts account for 3.4-8.3 % and 8.7-12.5 %, respectively, and the remaining transcripts combined account for < 1 % of the total acrosomal vesicle protein 1 message. It is suggested that phenomena of cryptic splicing and exon skipping occur within this gene. The acrosomal vesicle protein 1 may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans.
Alias Symbols: D11S4365, SP-10, SPACA2
Protein Size: 265

Molecular Weight: 29 kDa

Gene ID: 56

NCBI Accession: NM_001612, NP_001603

UniProt: P26436