ACRV1 antibody (N-Term)

Catalog No. ABIN2785464

Cited in 1 publication. The Rabbit Polyclonal anti-ACRV1 antibody (ABIN2785464) specifically detects ACRV1 in WB. The antibody is reactive with Human, Guinea Pig and Rabbit samples.

Quick Overview for ACRV1 antibody (N-Term) (ABIN2785464)

Target: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Reactivity: Human, Guinea Pig, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ACRV1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-ACRV1 Antibody

Binding Specificity: N-Term

Purpose: ACRV1 Antibody - N-terminal region

Sequence: MNRFLLLMSL YLLGSARGTS SQPNELSGSI DHQTSVQQLP GEFFSLENPS

Predicted Reactivity: Guinea Pig: 79%, Human: 100%, Rabbit: 85%

Characteristics: This is a rabbit polyclonal antibody against ACRV1. It was validated on Western Blot using a cell lysate as a positive control.

Purification: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ACRV1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

We also sell a specific blocking peptide that can be used in combination with this antibody. You can find the blocking peptide under ABIN8093830

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: prevent freeze-thaw cycles

Storage: 4 °C,-20 °C

Storage Comment: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-ACRV1 antibody (ABIN2785464)

Reddi, Shore, Acharya, Herr: "Transcriptional regulation of spermiogenesis: insights from the study of the gene encoding the acrosomal protein SP-10." in: Journal of reproductive immunology, Vol. 53, Issue 1-2, pp. 25-36, (2001) (PubMed).

Target Details for ACRV1

Target: ACRV1 (Acrosomal Vesicle Protein 1 (ACRV1))

Alternative Name: ACRV1

Background:

Background Information: ACRV1 is a testis-specific, differentiation antigen, acrosomal vesicle protein 1, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. The acrosomal vesicle protein 1 may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans. This gene encodes a testis-specific, differentiation antigen, acrosomal vesicle protein 1, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. This gene consists of 4 exons and its alternative splicing generates multiple distinct transcripts, which encode protein isoforms ranging from 81 to 265 amino acids. The longest transcript is the most abundant, comprising 53-72 % of the total acrosomal vesicle protein 1 messages, the second largest transcript comprises 15-32 %, the third and the fourth largest transcripts account for 3.4-8.3 % and 8.7-12.5 %, respectively, and the remaining transcripts combined account for < 1 % of the total acrosomal vesicle protein 1 message. It is suggested that phenomena of cryptic splicing and exon skipping occur within this gene. The acrosomal vesicle protein 1 may be involved in sperm-zona binding or penetration, and it is a potential contraceptive vaccine immunogen for humans.

Gene Name: Acrosomal vesicle protein 1

Alternative Symbols: SP-10, SPACA2, D11S4365

Protein Name: Acrosomal protein SP-10

Molecular Weight: 29kDa

Gene ID: 56

NCBI Accession: NP_001603

UniProt: P26436