MHC Class I RT1.Aa alpha-Chain (RT1.AA) antibody

Catalog No. ABIN120721

This Mouse Monoclonal antibody specifically detects MHC Class I RT1.Aa alpha-Chain in FACS, IHC (fro) and IP. It exhibits reactivity toward Rat.

Quick Overview for MHC Class I RT1.Aa alpha-Chain (RT1.AA) antibody (ABIN120721)

Target: MHC Class I RT1.Aa alpha-Chain (RT1.AA)

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoprecipitation (IP)

Clone: OX-18

Product Details

Purification: Protein G affinity chromatography

Immunogen: Rat spleen cell glycoprotein

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: 1:50-1:100, use 10 μL of the suggested working dilution to label 10^6 cells. Immunohistochemistry on frozen sections: Acetone fixation recommended - the antigen is sensitive to fixation with paraformaldehyde. Not suitable on paraffin embedded sections. ELISA. Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.09 % sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2-8 °C for up to one month or at -20 °C for longer. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details

Target: MHC Class I RT1.Aa alpha-Chain (RT1.AA)

Alternative Name: MHC Class I (RT1Aa)

Background: MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: RT1 class I histocompatibility antigen AA alpha chain