LIME antibody (Intracellular)

Catalog No. ABIN120743

The Mouse Monoclonal anti-LIME antibody has been validated for IP. It is suitable to detect LIME in samples from Human.

Quick Overview for LIME antibody (Intracellular) (ABIN120743)

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LIME antibody is un-conjugated

Application: Immunoprecipitation (IP)

Clone: LIME-06

Product Details anti-LIME Antibody

Binding Specificity: AA 141-295, Intracellular

Specificity: The antibody LIME-06 was raised against intracellular fragment corresponding to aa 141-295 of human LIME, a 31 kDa Lck-interacting transmembrane adaptor expressed by T cells.

Purification: Protein A affinity chromatography

Purity: > 95 % by SDS-PAGE

Immunogen: Bacterially expressed intracellular fragment corresponding to aa 141-295 of human LIME

Isotype: IgG1

Application Details

Application Notes: Suitable for Immunoprecipitation.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.4 containing 15 mM sodium azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for LIME

Target: LIME (LIME1) (Lck Interacting Transmembrane Adaptor 1 (LIME1))

Alternative Name: LIME1

Background: LIME (Lck-interacting molecule) is a 31 kDa double-palmitoylated protein with unusually basic cytoplasmic domain, expressed by T cells. After ligation of CD4 or CD8 T cell coreceptors, LIME is phosphorylated by Src-family kinases and associates with Lck and Fyn kinases and with their negative regulator Csk. Interestingly, Csk-mediated phosphorylation of C-terminal negative-regulatory tyrosine of LIME-associated Lck can result in increase of enzymatic activity compared with the total pool of Lck, thus, LIME serves as a positive regulator of TCR-dependent T cell signaling. However, under some circumstances, LIME may mediate inhibitory signals.Synonyms: LIME, LIME-1, LIME1, Lck-interacting membrane protein, Lck-interacting molecule, Lck-interacting transmembrane adapter 1

Gene ID: 54923

UniProt: Q9H400