TUBA1B antibody

Catalog No. ABIN121117

Product Details anti-TUBA1B Antibody

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Reactivity: Human, Mouse, Rat, Avian, Mammalian, Saccharomyces cerevisiae

Host: Rat

Clonality: Monoclonal

Conjugate: This TUBA1B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA), Immunoprecipitation (IP), Radioimmunoassay (RIA)

Specificity: SM568P specifically recognizes the alpha subunit of Tubulin. The reactivity pattern is similar to that seen with Clone YL1/2 (cat.-No SM2202P). This antibody is routinely tested in ELISA on Tubulin.

Purification: Affinity Chromatography on Protein G

Immunogen: Yeast tubulin

Clone: YOL1-34

Isotype: IgG2a

Application Details

Application Notes: Immunohistochemistry on Frozen Sections. ELISA: 10 μg/ul as detecting antibody. RIA. Western blotting.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This antibody is routinely tested in Elisa on Tubulin.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

References for anti-TUBA1B antibody (ABIN121117)

Lang, Grether-Beck, Singh, Kuck, Jakob, Kefalas, Altinoluk-Hambüchen, Graffmann, Schneider, Lindecke, Brenden, Felsner, Ezzahoini, Marini, Weinhold, Vierkötter, Tigges, Schmidt, Stühler, Köhrer et al.: "MicroRNA-15b regulates mitochondrial ROS production and the senescence-associated secretory phenotype through sirtuin 4/SIRT4. ..." in: Aging, Vol. 8, Issue 3, pp. 484-505, (2016) (PubMed).

Takeyama, Yuki, Tokuhara, Oroku, Mejima, Kurokawa, Kuroda, Kodama, Nagai, Ueda, Kiyono: "Oral rice-based vaccine induces passive and active immunity against enterotoxigenic E. coli-mediated diarrhea in pigs." in: Vaccine, Vol. 33, Issue 39, pp. 5204-11, (2016) (PubMed).

Kessler, Reich, Jahn, Tolosa, Beck, Kalbacher, Overkleeft, Schempp, Driessen: "Human cytomegalovirus infection interferes with major histocompatibility complex type II maturation and endocytic proteases in dendritic cells at multiple levels." in: The Journal of general virology, Vol. 89, Issue Pt 10, pp. 2427-36, (2008) (PubMed).

Valenta, Lukas, Doubravska, Fafilek, Korinek: "HIC1 attenuates Wnt signaling by recruitment of TCF-4 and beta-catenin to the nuclear bodies." in: The EMBO journal, Vol. 25, Issue 11, pp. 2326-37, (2006) (PubMed).

Target Details for TUBA1B

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Alternative Name: alpha Tubulin / TUBA1B (TUBA1B Products)

Synonyms: RGD1565476 antibody, K-ALPHA-1 antibody, cb944 antibody, fb22g06 antibody, tuba1 antibody, wu:fb22g06 antibody, Tuba2 antibody, TUBA3 antibody, k-alpha-1 antibody, tuba antibody, Calpha1 antibody, Tuba1 antibody, TUBA1B antibody, tubulin, alpha 1B antibody, tubulin alpha 1b antibody, tubulin, alpha 1b antibody, tubulin alpha 1b L homeolog antibody, tubulin alpha-1B chain antibody, Tubulin alpha-1B chain antibody, Tuba1b antibody, TUBA1B antibody, tuba1b antibody, tuba1b.L antibody, LOC100445003 antibody, LOC102178700 antibody

Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.Synonyms: Alpha-tubulin ubiquitous, Tubulin K-alpha-1, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain

Pathways: Microtubule Dynamics, M Phase