GAD antibody (C-Term)

Catalog No. ABIN121539

The Rabbit Polyclonal anti-GAD antibody (ABIN121539) specifically detects GAD in WB, IHC (p), IHC (fro) and EIA. The antibody is reactive with Human, Mouse, Rat and Cat samples.

Quick Overview for GAD antibody (C-Term) (ABIN121539)

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Reactivity: Human, Mouse, Rat, Cat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GAD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details anti-GAD Antibody

Binding Specificity: C-Term

Cross-Reactivity (Details): Also reacts to: GAD2 / GAD65

Characteristics: Synonyms: Glutamate decarboxylase 1, GAD-67, Glutamate decarboxylase 67 kDa isoform, 67 kDaglutamic acid decarboxylase

Purification: Ammonium Sulphate Precipitation.

Immunogen: Synthetic peptide sequence from the C-terminus of GADAA Sequence: D-F-L-I-E-E-I-E-R-L-G-Q-D-L

Application Details

Application Notes: ELISA. Western Blot: 1/1000-1/10000. Immunohistochemistry on Frozen Sections: 1/200-1/5000. Recommended Positive Control: Brain.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GAD

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Alternative Name: GAD1 / GAD67

Background: This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma aminobutyric acid from L glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin dependent diabetes. This gene may also play a role in the stiff man syndrome.Synonyms: 67 kDa glutamic acid decarboxylase, GAD-67, Glutamate decarboxylase 1, Glutamate decarboxylase 67 kDa isoform

Gene ID: 2571

UniProt: Q99259