FEN1 antibody (N-Term)

Catalog No. ABIN121840

This Rabbit Polyclonal antibody specifically detects FEN1 in WB and IP. It exhibits reactivity toward Human.

Quick Overview for FEN1 antibody (N-Term) (ABIN121840)

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FEN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-FEN1 Antibody

Binding Specificity: N-Term

Specificity: This antibody is specific for Flap Structure-specific Endonuclease 1 (FEN1).

Purification: Affinity Chromatography.

Immunogen: Immunogen Synthetic peptide corresponding to a region within the N terminal domain of human FEN1 (locus link ID 2237)

Application Details

Application Notes: Western Blot: 1:5000 - 1:30000, this antibody detects a band of approximately 42 kD in Hela cell lysates. Immunoprecipitation: 4 μg/mg protein - 10 μg/mg protein.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: TRIS-Citrate/Phosphate pH 7.5 containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Alternative Name: FEN1 / RAD2

Background: FEN1 is a member of the XPG/RAD2 endonuclease family which has an important role in lagging strand DNA synthesis and base excision repair. It is hypothesised that FEN1 may play a role in the inhibltion of triplet repeat expansion.Synonyms: DNase IV, FEN-1, Flap endonuclease 1, Flap structure-specific endonuclease 1, MF1, Maturation factor 1

Gene ID: 2237

UniProt: P39748

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA