FEN1 antibody (AA 50-380)

Catalog No. ABIN3021632

The Rabbit Polyclonal anti-FEN1 antibody is suitable to detect FEN1 in samples from Human. It has been validated for WB, IHC, IF and IP.

Quick Overview for FEN1 antibody (AA 50-380) (ABIN3021632)

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FEN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Product Details anti-FEN1 Antibody

Binding Specificity: AA 50-380

Sequence: GDVLQNEEGE TTSHLMGMFY RTIRMMENGI KPVYVFDGKP PQLKSGELAK RSERRAEAEK QLQQAQAAGA EQEVEKFTKR LVKVTKQHND ECKHLLSLMG IPYLDAPSEA EASCAALVKA GKVYAAATED MDCLTFGSPV LMRHLTASEA KKLPIQEFHL SRILQELGLN QEQFVDLCIL LGSDYCESIR GIGPKRAVDL IQKHKSIEEI VRRLDPNKYP VPENWLHKEA HQLFLEPEVL DPESVELKWS EPNEEELIKF MCGEKQFSEE RIRSGVKRLS KSRQGSTQGR LDDFFKVTGS LSSAKRKEPE PKGSTKKKAK TGAAGKFKRG K

Cross-Reactivity: Human, Mouse

Characteristics: Polyclonal Antibodies

Purification: Affinity purification

Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-380 of human FEN-1 (NP_004102.1).

Isotype: IgG

Application Details

Application Notes: WB,1:500 - 1:2000,IHC,1:50 - 1:200,IF,1:10 - 1:100,IP,1:50 - 1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide,50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid freeze / thaw cycles

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for FEN1

Target: FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))

Alternative Name: FEN1

Background: The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.,FEN1,FEN-1,MF1,RAD2,Epigenetics & Nuclear Signaling,DNA Damage & Repair,FEN1

Molecular Weight: 35 kDa/42 kDa

Gene ID: 2237

UniProt: P39748

Pathways: Telomere Maintenance, DNA Damage Repair, DNA Replication, Synthesis of DNA