CXCR5 antibody (Cytoplasmic Domain)

Catalog No. ABIN121946

The Rabbit Polyclonal anti-CXCR5 antibody is suitable to detect CXCR5 in samples from Human. It has been validated for IHC (p).

Quick Overview for CXCR5 antibody (Cytoplasmic Domain) (ABIN121946)

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCR5 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CXCR5 Antibody

Binding Specificity: Cytoplasmic Domain

Specificity: Recognizes Human CXC Chemokine Receptor 5 (CXCR5) (family: GPCR, subfamily: Chemokine). BLAST analysis of the peptide immunogen showed no homology with other Human proteins, except MTA3 (50 %).

Cross-Reactivity (Details): Species reactivity (expected):Hamster, Mouse, Pig, Rabbit, Rat.
Species reactivity (tested):Human, Gibbon, Gorilla.

Purification: Peptide Immunoaffinity Chromatography

Immunogen: Synthetic peptide - KLH conjugated.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry with formalin fixed paraffin embedded tissues: 5-18 μg/mL. Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.7, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-80 °C

Storage Comment: Store the antibody at 2-8 °C for up to one month or at -70 °C for longer. Avoid repeatedfreezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CXCR5

Target: CXCR5 (Chemokine (C-X-C Motif) Receptor 5 (CXCR5))

Alternative Name: CD185 / CXCR5

Background: CXCR5, a Chemokine Receptor, is required for normal development of Peyer's patches, inguinal lymph nodes, and splenic follicles. CXCR5 binds B-lymphocyte chemoattractant (BLC) and is involved in B-cell homing to follicles in lymph nodes and spleen. Two isoforms are produced by alternative splicing. CXCR5 expression has been reported in human blood and salivary gland and in animal lymphatic organs, blood, bone marrow, brain, liver, lymph node, skin, spleen, stomach, and tonsil. ESTs have been isolated from human brain, germ cell, lung, lymph, pancreas, skin, stomach, and uterus cancer libraries, and from normal human blood, liver/spleen, lymph node, nerve, placenta, small intestine, tonsil, and B-cell/lung/testis libraries.Synonyms: BLR1, Burkitt lymphoma receptor 1, C-X-C chemokine receptor type 5, CXC-R5, CXCR-5, MDR15, Monocyte-derived receptor 15

Gene ID: 643

UniProt: P32302