Serotonin Receptor 4 antibody (Cytoplasmic Domain)

Catalog No. ABIN121987

Product Details anti-Serotonin Receptor 4 Antibody

Target: Serotonin Receptor 4 (HTR4)

Binding Specificity: Cytoplasmic Domain

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 4 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: Reacts with 5-HT4 Receptor (family: GPCR, subfamily: Serotonin).

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Immunohistochemistry with Formalin Fixed Paraffin Embedded Tissues: 6 μg/mL. Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 to -70 °C forlonger. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Serotonin Receptor 4

Target: Serotonin Receptor 4 (HTR4)

Alternative Name: Serotonin Receptor 4 / HTR4 (HTR4 Products)

Background: The 5-HT4 receptor, a member of the Serotonin Receptor subfamily, has been shown to affect the sensory and motor functions of the gut. Some of the most promising emerging therapies in Irritable Bowel Syndrome revolve around targeted pharmacotherapeutic modulation of serotonin receptors (ie, 5-HT3 and 5-HT4 subtypes). At least 9 isoforms (5-HT4A-5-HT4G, and 5-HT4n) have been described, which differ in the length and composition of their intracellular C termini after a common splicing site (L358). A ninth isoform (5-HT4H) results in the insertion of 14 amino acids into the second intracellular loop. 5-HT4 receptor expression has been documented primarily in the brain, and in colon, heart, and vessel. An EST has been isolated from a human cancerous nerve library. Ligand: Serotonin.Synonyms: 5-HT-4, 5-HT4, 5-hydroxytryptamine receptor 4

Gene ID: 3360

UniProt: Q13639

Pathways: JAK-STAT Signaling, cAMP Metabolic Process, S100 Proteins