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Serotonin Receptor 4 antibody (C-Term)

HTR4 Reactivity: Human, Cow, Dog, Horse, Guinea Pig, Rabbit, Bat, Hamster IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1048274
  • Target See all Serotonin Receptor 4 (HTR4) Antibodies
    Serotonin Receptor 4 (HTR4)
    Binding Specificity
    • 15
    • 15
    • 13
    • 10
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    C-Term
    Reactivity
    • 61
    • 34
    • 33
    • 10
    • 9
    • 9
    • 6
    • 5
    • 5
    • 4
    • 4
    • 3
    • 1
    • 1
    Human, Cow, Dog, Horse, Guinea Pig, Rabbit, Bat, Hamster
    Host
    • 75
    • 1
    Rabbit
    Clonality
    • 75
    • 1
    Polyclonal
    Conjugate
    • 29
    • 7
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    This Serotonin Receptor 4 antibody is un-conjugated
    Application
    • 61
    • 27
    • 26
    • 26
    • 12
    • 9
    • 6
    • 6
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human 5HT4 Receptor. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit, Guinea pig (100%) Marmoset, Elephant, Opossum, Turkey, Chicken, Platypus, Lizard (94%) Mouse, Rat (88%) Pig (82%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 17 amino acid peptide from C-terminus of human 5HT4 Receptor. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Hamster, Panda, Dog, Bat, Bovine, Horse, Rabbit, Guinea pig (100%), Marmoset, Elephant, Opossum, Turkey, Chicken, Platypus, Lizard (94%), Mouse, Rat (88%), Pig (82%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product HTR4 Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (13 μg/mL)
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    Serotonin Receptor 4 (HTR4)
    Alternative Name
    HTR4 / 5-HT4 Receptor (HTR4 Products)
    Synonyms
    5-HT4 antibody, 5-HT<4L> antibody, 5HTR4 antibody, 5-HT4S antibody, 5-HT4R antibody, 5 hydroxytryptamine (serotonin) receptor 4 antibody, 5-hydroxytryptamine receptor 4 antibody, Htr4 antibody, HTR4 antibody
    Background
    Name/Gene ID: HTR4
    Subfamily: Serotonin
    Family: GPCR

    Synonyms: HTR4, 5-HT4, 5-HT4R, 5-HT-4, 5HT4 receptor, 5-HT4 receptor, 5-hydroxytryptamine 4 receptor, 5-hydroxytryptamine receptor 4, Serotonin 5-HT-4a receptor, Serotonin receptor 4, Cardiac 5-HT4 receptor, Serotonin 4 receptor, Serotonin 5-HT-4 receptor
    Gene ID
    3360
    Pathways
    JAK-STAT Signaling, cAMP Metabolic Process, S100 Proteins
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