GPR109B antibody

Catalog No. ABIN122203

This Rabbit Polyclonal antibody specifically detects GPR109B in IF and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for GPR109B antibody (ABIN122203)

Target: GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPR109B antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GPR109B Antibody

Specificity: Reacts with G Protein-Coupled Receptor HM74 (family: GPCR, subfamily: Nicotinic Acid)

Purification: Peptide immunogen affinity column

Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Immunocytochemistry. Immunohistochemistry on formalin fixed paraffin embedded tissues: 19-38 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, pH 7.7, containing 0.01 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-80 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for GPR109B

Target: GPR109B (HCAR3) (Hydroxycarboxylic Acid Receptor 3 (HCAR3))

Alternative Name: Nicotinic Acid Receptor 2

Background: GPR109B/HM74 is a Nicotinic Acid GPCR. HM74 expression has been reported in adipose and spleen, as well as lymphocytes and monocytes. ESTs have been isolated from adipose, blood, liver, liver/spleen, placenta, prostate, and testis libraries.Synonyms: G-protein coupled receptor 109B, GPR109B, HM74, HM74B, NIACR2, Niacin receptor 2

Gene ID: 8843

UniProt: P49019