Metabotropic Glutamate Receptor 1 antibody (Extracellular Domain)

Catalog No. ABIN122309

The Rabbit Polyclonal anti-Metabotropic Glutamate Receptor 1 antibody has been validated for IHC (p). It is suitable to detect Metabotropic Glutamate Receptor 1 in samples from Human, Mouse and Rat.

Quick Overview for Metabotropic Glutamate Receptor 1 antibody (Extracellular Domain) (ABIN122309)

Target: Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Metabotropic Glutamate Receptor 1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Metabotropic Glutamate Receptor 1 Antibody

Binding Specificity: Extracellular Domain

Specificity: Reacts with Metabotropic Glutamate Receptor 1 (family: GPCR, subfamily: Metabotropic glutamate)

Purification: Peptide immunogen affinity column

Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Immunohistochemistry with formalin fixed paraffin embedded tissues: 17 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS, pH 7.7, containing 0.01 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-80 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for Metabotropic Glutamate Receptor 1

Target: Metabotropic Glutamate Receptor 1 (GRM1) (Glutamate Receptor, Metabotropic 1 (GRM1))

Alternative Name: mGluR1a / GRM1a

Background: The Metabotropic Glutamate Receptor mGluR1 binds L-glutamate, the major excitatory neurotransmitter in the central nervous system, which activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. mGluR1 and mGluR5 are group I receptors that have been shown to activate phospholipase C. Alternative splice variants of the mGluR1 gene have been described, but their full-length nature has not been determined. Inflammation results in the activation of mGluR1 and mGluR5 in dorsal horn neurons of the spinal cord, leading to extracellular signal-regulated kinase (ERK) activation, which is required for nociceptive plasticity and enhanced pain. mGluR1 has been reported in various regions of the brain and spinal cord. ESTs have been isolated from brain and eye libraries. Caution: GLUR1 refers to Ionotropic Glutamate Receptor 1, not to Metabotropic Glutamate Receptor 1 (mGLUR1).Synonyms: Gprc1a, Metabotropic glutamate receptor 1, Mglur1

UniProt: Q13255