TNIK antibody

Catalog No. ABIN122435

The Rabbit Polyclonal anti-TNIK antibody is suitable to detect TNIK in samples from Human. It has been validated for IHC (p).

Quick Overview for TNIK antibody (ABIN122435)

Target: TNIK (TRAF2 and NCK Interacting Kinase (TNIK))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNIK antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-TNIK Antibody

Specificity: Reacts with Traf2 and NCK-Interacting Kinase (TNIK) (Family: Protein Kinase, Subfamily: MSN)

Cross-Reactivity (Details): Species reactivity (expected):Mouse, RatSpecies reactivity (tested):Human

Purification: Peptide immunogen affinity column

Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Immunocytochemistry. Immunohistochemistry with formalin fixed paraffin embedded tissues: 2 μg/mL Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, pH 7.7, containing 0.01 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-80 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -70 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for TNIK

Target: TNIK (TRAF2 and NCK Interacting Kinase (TNIK))

Alternative Name: TNIK

Background: TNIK is a MSN protein kinase that interacts with both TNF receptor-associated factor 2 (TRAF2) and the adapter protein NCK. The protein has been shown to activate the c-Jun N-terminal kinase pathway when overexpressed in Phoenix-A cells, and this activation is mediated solely by the C-terminal germinal center kinase homology region. TNIK has been shown to phosphorylate gelsolin, the principal intracellular and extracellular actin-severing protein, in vitro. This and evidence from mutational studies suggest that TNIK functions in the regulation of the cytoskeleton. At least eight splice isoforms have been reported. Northern analysis indicates TNIK expression in human heart, skeletal muscle, and brain, with lower levels of expression in kidney, liver, lung, and pancreas. ESTs have been isolated from human tissue libraries, including normal amnion, gallbladder, skin, and testis and cancerous bone, germ cell, nerve, and placenta.Synonyms: KIAA0551, TRAF2 and NCK-interacting protein kinase

Gene ID: 23043

UniProt: Q9UKE5