GRPR antibody (Cytoplasmic Domain)

Catalog No. ABIN122617

This Rabbit Polyclonal antibody specifically detects GRPR in IHC (p) and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for GRPR antibody (Cytoplasmic Domain) (ABIN122617)

Target: GRPR (Gastrin-Releasing Peptide Receptor (GRPR))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRPR antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-GRPR Antibody

Binding Specificity: Cytoplasmic Domain

Specificity: Reacts with Gastrin-releasing peptide receptor at 3rd cytoplasmic domain. BLAST analysis of the peptide immunogen showed no homology with other Human proteins, except ADAM28 (41 %).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat (see 'Immunogen Remarks').
Species reactivity (tested):Human.

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide - KLH conjugated

Application Details

Application Notes: Immunocytochemistry: 5 μg/mL. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin embedded sections. Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0, at 99-100 °C for 20 min. Remove from heat and let stand at room temperature in buffer for 20 min. Rinse in 1x TBS with Tween (TBST) for 1 min. at room temperature.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: This product was originally produced by MBL International.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C forlonger. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for GRPR

Target: GRPR (Gastrin-Releasing Peptide Receptor (GRPR))

Alternative Name: Gastrin-Releasing Peptide Receptor

Background: Gastrin-releasing peptide receptor (GRPR) is a bombesin receptor. In addition to its involvement in autism and mental retardation, GRPR may also play a role in the progression of cancer. Nicotine exposure induces an increase in GRPR expression in the lung. GRPR has been reported in brain, breast, colon, lung, lymph node, ovary, placenta, prostate, stomach, and uterus. It has also been shown to be expressed in several cancers, including those in breast, colon, lung, ovary, pancreas, and prostate.Synonyms: GRP-R, GRP-preferring bombesin receptor, GRPR

Gene ID: 2925

UniProt: P30550