Cytokeratin 19 antibody (Biotin)

Catalog No. ABIN125675

The Mouse Monoclonal anti-Cytokeratin 19 antibody has been validated for WB, FACS, IHC (p), ICC and IP. It is suitable to detect Cytokeratin 19 in samples from Human. There are 3+ publications available.

Quick Overview for Cytokeratin 19 antibody (Biotin) (ABIN125675)

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 19 antibody is conjugated to Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Clone: BA-17

Product Details anti-Cytokeratin 19 Antibody (Biotin)

Purpose: Anti-Cytokeratin 19 Biotin

Specificity: The antibody BA-17 reacts with cytokeratin 19 (40 kDa intracellular antigen) in human tissue. Cytokeratin 19 is not expressed in hepatocytes, it is often co-expressed with cytokeratin 7.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogen: Mammary organoids.

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: Recommended dilution: 1-2 μg/mL.
Flow cytometry: Recommended dilution: 4-10 μg/mL. Intracellular staining.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-Cytokeratin 19 antibody (Biotin) (ABIN125675)

Lewis, Klimstra, Varmus: "The c-myc and PyMT oncogenes induce different tumor types in a somatic mouse model for pancreatic cancer." in: Genes & development, Vol. 17, Issue 24, pp. 3127-38, (2003) (PubMed).

Bartek, Durban, Hallowes, Taylor-Papadimitriou: "A subclass of luminal epithelial cells in the human mammary gland, defined by antibodies to cytokeratins." in: Journal of cell science, Vol. 75, pp. 17-33, (1985) (PubMed).

Bartek, Taylor-Papadimitriou, Miller, Millis: "Patterns of expression of keratin 19 as detected with monoclonal antibodies in human breast tissues and tumours." in: International journal of cancer. Journal international du cancer, Vol. 36, Issue 3, pp. 299-306, (1985) (PubMed).

Target Details for Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Alternative Name: Cytokeratin 19

Background: Keratin 19,Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.,K19, CK19, CYK19, KRT19, K1CS

Gene ID: 3880

UniProt: P08727