Transferrin antibody

Catalog No. ABIN125745

Cited in 1 publication. The Mouse Monoclonal anti-Transferrin antibody (Clone PTF-02) (ABIN125745) specifically detects Transferrin in WB and ELISA. The antibody is reactive with Pig samples.

Quick Overview for Transferrin antibody (ABIN125745)

Target: Transferrin (TF)

Reactivity: Pig

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: PTF-02

Product Details anti-Transferrin Antibody

Purpose: Anti-Por Transferrin Purified

No Cross-Reactivity: Horse, Sheep

Cross-Reactivity (Details): Porcine

Characteristics: The antibody PTF-02 recognizes porcine serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Porcine transferrin

Isotype: IgG1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-Transferrin antibody (ABIN125745)

Trebichavský, Mack?, Viklický, Koukal, Siman: "Monoclonal antibodies against pig transferrin. Blocking and binding activity." in: Folia microbiologica, Vol. 32, Issue 5, pp. 448-52, (1988) (PubMed).

Target Details for Transferrin

Target: Transferrin (TF)

Alternative Name: Transferrin

Background: Transferrin,Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.,TFQTL1, PRO1557, PRO2086

Gene ID: 396996

UniProt: P09571

Pathways: Transition Metal Ion Homeostasis