HSP70 antibody (C-Term)

Catalog No. ABIN125828

The Rabbit Polyclonal anti-HSP70 antibody is suitable to detect HSP70 in samples from Rainbow Trout and Zebrafish (Danio rerio). It has been validated for WB, IHC and IP.

Quick Overview for HSP70 antibody (C-Term) (ABIN125828)

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reactivity: Rainbow Trout, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-HSP70 Antibody

Binding Specificity: C-Term

Purpose: Anti-HSP70 | Salmonid heat shock protein 70, Affinity purified

Cross-Reactivity (Details): Oncorhynchus mykiss (Rainbow trout), Danio rerio (Zebrafish)
Not reactive in: Gasterosteus aculeatus

Predicted Reactivity: Coregonus clupeaformis,Oncorhynchus mykiss,Salmo salar (Atlantic salmon),Salvelinus fontinalis (Brook trout)

Purification: Immunogen affinity purified serum

Immunogen: KLH-conjugated synthetic peptide chosen from the C-terminal of salmonid hsp70. The target peptide is a sequence specific to salmonid hsp70 UniProt: B5X4Z3.

Application Details

Application Notes: 5 μg of antibodies in reaction mixture (IP), 1 : 100 (IHC), 1 : 15 000 (WB)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 200 μL of sterile water

Buffer: Immunogen affinity purified serum in PBS pH 7.4.

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

Target Details for HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Alternative Name: HSP70

Background:

Background: Heat-shock protein 70 (Hsp70) is the major stress-inducible protein in vertebrates and highly conserved throughout evolution. It plays a role as a molecular chaperone and is important for allowing cells to cope with acute stressor insult, especially those affecting the protein machinery.

Additional information: Immunohistochemistry experiments have been done on salmon tissue treated with hydrated autoclaving of formalin fixed sections (unpublished results)

Molecular Weight: 70 kDa

Gene ID: 100194521

UniProt: B5X4Z3