Peroxiredoxin Q, Thioredoxin Dependent Peroxidase Chloroplast (PRXQ) antibody

Details for Product No. ABIN125852
Antigen
Reactivity
Arabidopsis thaliana, Maize/Corn (Zea mays), Manihot esculenta, Spinach (Spinacia oleracea)
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
Options
Immunogen His-tagged full length protein (with presequence) of Arabidopsis thaliana was overexpressed in in E.coli. Isolated with HiTrap column (GE Healthcare) Q9LU86, At3g26060
Cross-Reactivity (Details) Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
Predicted Reactivity Populus spp. , monocots including Triticum aestivum, Oryza sativa
Characteristics Expected / apparent Molecular Weight of the Antigene: 16 kDA
Purification serum
Antigen
Alternative Name PrxQ
Background AGI Code: At3g26060
Peroxiredoxins (EC=1.11.1.15) belong to the enzyme family which is ubiquitous in all kingdoms of life. Prx Q enzyme acting by reducing hydroperoxides. Peroxiredoxins have no heme group, unlike the other peroxidases, but perform their enzymatic activity using cysteine residues with redox-active thiol groups. The ability of peroxiredoxins to hydrolyze hydroperoxides suggests that this protein family has a general function in oxidant defence.
Molecular Weight 16 kDA
UniProt Q9LU86
Application Notes 1: 5000 with standard ECL (WB)
Comment

In stroma fractions a weak background reaction at 28 kDa is visible. No crossreactivity in any thylakoid fractions.

Restrictions For Research Use only
Format Lyophilized
Reconstitution For reconstitution add 200 μL of sterile water.
Handling Advice Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
Storage -20 °C
Storage Comment store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Background publications An, Fan, Li, Li, Li, Zhu, Wen, Carvalho, Chen: "Comparison of leaf proteomes of cassava (Manihot esculenta Crantz) cultivar NZ199 diploid and autotetraploid genotypes." in: PLoS ONE, Vol. 9, Issue 4, pp. e85991, 2014 (PubMed).

Wu, Wang, Chen, Wang, Wu, Zu, Chen: "Proteomic and phytohormone analysis of the response of maize (Zea mays L.) seedlings to sugarcane mosaic virus." in: PLoS ONE, Vol. 8, Issue 7, pp. e70295, 2013 (PubMed).

Product cited in: Wu, Han, Wang, Wang, Sun, Zu, Chen: "Comparative proteomic analysis of the plant-virus interaction in resistant and susceptible ecotypes of maize infected with sugarcane mosaic virus." in: Journal of proteomics, Vol. 89, pp. 124-40, 2013 (PubMed).

Pulido, Spuinola, Kirchsteiger, Guinea, Pascual, Sahrawy, Sandalio, Dietz, Gonzualez, Cejudo: "Functional analysis of the pathways for 2-Cys peroxiredoxin reduction in Arabidopsis thaliana chloroplasts." in: Journal of experimental botany, 2010 (PubMed).

Lehtima: "Nodularin uptake and induction of oxidative stress in spinach (Spinachia oleracea)." in: Journal of plant physiology, 2010 (PubMed).

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