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Ftsh9 antibody

This Rabbit Polyclonal antibody specifically detects Ftsh9 in WB. It exhibits reactivity toward Arabidopsis thaliana.
Catalog No. ABIN125875

Quick Overview for Ftsh9 antibody (ABIN125875)

Target

Ftsh9 (Plant Fts Metallo-Protease H9 (Ftsh9))

Reactivity

Arabidopsis thaliana

Host

Rabbit

Clonality

Polyclonal

Application

Western Blotting (WB)
  • Cross-Reactivity (Details)

    Not reactive in: no confirmed exceptions from predicted reactivity known in the moment

    Predicted Reactivity

    Arabidopsis thaliana

    Characteristics

    Expected / apparent Molecular Weight of the Antigene: 87.8 / 74.36 kDa

    Purification

    serum

    Immunogen

    KLH-conjugated synthetic peptide derived from Arabidopsis thaliana FtsH9 protein sequence Q9FIM2, At5g58870
  • Application Notes

    1: 500 -1: 1000 with standard ECL (WB)

    Comment

    Antibody recommended to be used on chloroplast and thylakoid samples. This antibody will not work with Chlamydomonas reinhardtii.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    For reconstitution add 182 μL of sterile water.

    Buffer

    PBS pH 7.4

    Handling Advice

    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

    Storage

    -20 °C

    Storage Comment

    store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
  • Target

    Ftsh9 (Plant Fts Metallo-Protease H9 (Ftsh9))

    Background

    AGI Code: At5g58870
    FtsH protease is important in chloroplast biogenesis and thylakoid maintenance. Nuclear genome of Arabidopsis thaliana contains 12 genes encoding FtsH proteins. Three of those gene products in Arabidopsis , can be targeted to mitochondria (FTSH3, FTSH4, and FTSH10), while the other nine (FTSH1, FTSH2, FTSH5 to FTSH9, FTSH11, and FTSH12) can enter the chloroplast. Synonyme: cell division protease FtsH homolog 9, chloroplastic.

    Molecular Weight

    expected: 87.8 kDa, apparent: 74.36 kDa

    UniProt

    Q9FIM2
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