PsaA (N-Term) antibody

Catalog No. ABIN125891

There are 5+ publications for this product available. The Rabbit Polyclonal anti-PsaA antibody is suitable to detect PsaA in samples from Marchantia polymorpha, Lolium perenne, Nannochloropsis gaditana, Nannochloropsis oceanica, Nicotiana benthamiana, Nicotiana tabacum, Oryza sativa, Pea, Physcomytrium Patens, Picea abies, Pilosella officinarum, Pinus strobus, Sinapis alba, Spinach, Synechococcus sp., Synechocystis, Syntrichia ruralis, Tetradesmus obliquus, Tillandsia flabellata, Ulva prolifera, Cytisus cantabricus, Barley, Begonia, Bryopsis corticulans, Chlamydomonas, Chlamydomonas reinhardtii, Chlorella sorokiniana, Chlorella vulgaris, Chromochloris zofingiensis, Colobanthus quitensis, Craterostigma pumilum, Arabidopsis thaliana, Dianthus caryophyllus, Drosera capensis, Euonymus japonicus, Fraxinus ornus, Fucus vesiculosus, Green bean, Haematococcus lacustris, Halomicronema hongdechloris and Lasallia hispanica. It has been validated for WB, BN PAGE and EM.

Quick Overview for PsaA (N-Term) antibody (ABIN125891)

Target: PsaA

Reactivity: Marchantia polymorpha, Lolium perenne, Nannochloropsis gaditana, Nannochloropsis oceanica, Nicotiana benthamiana, Nicotiana tabacum, Oryza sativa, Pea, Physcomytrium Patens, Picea abies, Pilosella officinarum, Pinus strobus, Sinapis alba, Spinach, Synechococcus sp., Synechocystis, Syntrichia ruralis, Tetradesmus obliquus, Tillandsia flabellata, Ulva prolifera, Cytisus cantabricus, Barley, Begonia, Bryopsis corticulans, Chlamydomonas, Chlamydomonas reinhardtii, Chlorella sorokiniana, Chlorella vulgaris, Chromochloris zofingiensis, Colobanthus quitensis, Craterostigma pumilum, Arabidopsis thaliana, Dianthus caryophyllus, Drosera capensis, Euonymus japonicus, Fraxinus ornus, Fucus vesiculosus, Green bean, Haematococcus lacustris, Halomicronema hongdechloris, Lasallia hispanica

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Blue-native PAGE (BN PAGE), Electron Microscopy (EM)

Product Details

Binding Specificity: N-Term

Purpose: Anti-PsaA | PSI-A core protein of photosystem I

Cross-Reactivity (Details): Arabidopsis thaliana, Begonia sp. , Bryopsis corticulans, Chlamydomonas reinhardtii, psychrophilic Chlamydomonas sp. UWO241 and Chlamydomonas sp. ICE-MDV, Chlorella sorokiniana, Chlorella vulgaris, Chromochloris zofingiensis, Colobanthus quitensis Kunt Bartl, Craterostigma pumilum, Cytisus cantabricus (Wilk.) Rchb. F., Dianthus caryophyllus, Dioxoniella giordanoi (red alga), Drosera capensis, Euonymus japonicus, Fraxinus ornus, Fucus vesiculosus, Haematococcus pluvialis, Halomicronema hongdechloris, Hieracium pilosella L., Hordeum vulgare, Lasallia hispanica, Lolium perenne, Nannochloropsis oceanica strain IMET1, Nicotiana benthamiana, Nicotiana tabacum, Oryza sativa, Pisum sativum, Marchantia polymorpha (liverwort), micro Nannochloropsis gaditana, Phaseolus vulgaris, Physcomitrium patens, Picea abies, Pinus strobus, Sinapsis alba, Spinacia oleracea, Synechococcus PCC 7942, Synechocystis PCC 6803, Syntrichia muralis (Hedw.) Raab, Scenedesmus obliquus, Tillandsia flabellate, Ulva prolifera
Not reactive in: Chromera velia

Predicted Reactivity: Algae,Bigelowiella natans,Cannabis sativa,Catalpa bungei,Citrus x limon,Cyanobacteria,Cyanidioschyzon merolae strain 10D,Galdieria sulphuraria,Lycopersicum esculentum,Panax ginseng,Picea spinulosa,Pinus thunbergii,Phaeodactylum tricornutum,Populus alba,Thermosynechococcus elongatus (strain BP-1),Triticum aestivum

Purification: Serum

Immunogen: N-terminal part of recombinant PsaA protein from Chlamydomonas reinhardtii P12154

Application Details

Application Notes: 1 : 20 (IG), 1 : 1000-1 : 5000 (WB)

Comment:

immunogold localization has been done in leaf material of Arabidopsis thaliana

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 μL of sterile water

Buffer: Serum

Handling Advice: Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube

Storage: -20 °C

Storage Comment: Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.

References

Simionato, Block, La Rocca, Jouhet, Maréchal, Finazzi, Morosinotto: "The response of Nannochloropsis gaditana to nitrogen starvation includes de novo biosynthesis of triacylglycerols, a decrease of chloroplast galactolipids, and reorganization of the photosynthetic apparatus." in: Eukaryotic cell, Vol. 12, Issue 5, pp. 665-76, (2013) (PubMed).

Ueda, Kuniyoshi, Yamamoto, Sugimoto, Ishizaki, Kohchi, Nishimura, Shikanai: "Composition and physiological function of the chloroplast NADH dehydrogenase-like complex in Marchantia polymorpha." in: The Plant journal : for cell and molecular biology, Vol. 72, Issue 4, pp. 683-93, (2012) (PubMed).

Godman, Molna: "RNA silencing of hydrogenase(-like) genes and investigation of their physiological roles in the green alga Chlamydomonas reinhardtii." in: The Biochemical journal, (2010) (PubMed).

Teardo, Formentin, Segalla, Giacometti, Marin, Zanetti, Schiavo, Zoratti, Szaba: "Dual localization of plant glutamate receptor AtGLR3.4 to plastids and plasmamembrane." in: Biochimica et biophysica acta, (2010) (PubMed).

Pollari, Rantama: "Effects of Deficiency and Overdose of Group 2 Sigma Factors in Triple Inactivation Strains of Synechocystis sp. Strain PCC 6803." in: Journal of bacteriology, Vol. 193, Issue 1, pp. 265-73, (2010) (PubMed).

Target Details

Target: PsaA

Background:

Background: PsaA is a core protein of photosystem I. In plants and cyanobacteria, the primary step in oxygenic photosynthesis, the light induced charge separation, is driven bytwo large membrane intrinsic protein complexes, the photosystems I and II. Synonym: Photosystem I P700 chlorophyll a apoprotein A1.

Additional information: PsaA is a hydrophobic protein and we recommend to use PVDF membrane for transfer to assure best results.This product can be sold containing ProClin if requested.

Molecular Weight: 82 | 55-60 kDa

Gene ID: 2717000

UniProt: P12154