PsaA (N-Term) antibody
Reactivity: Marchantia polymorpha, Nicotiana tabacum, Oryza sativa, Arabidopsis thaliana, Scenedesmus obliquus, Barley, Spinach, Chlamydomonas reinhardtii, Synechococcus PCC 7942, Fucus vesiculosus, Synechocystis PCC 6803, Green bean
EM, WB
Host: Rabbit
Polyclonal
unconjugated
1 image
(5 references)-
- Target
- PsaA
- Binding Specificity
- N-Term
- Reactivity
- Marchantia polymorpha, Nicotiana tabacum, Oryza sativa, Arabidopsis thaliana, Scenedesmus obliquus, Barley, Spinach, Chlamydomonas reinhardtii, Synechococcus PCC 7942, Fucus vesiculosus, Synechocystis PCC 6803, Green bean
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Electron Microscopy (EM), Western Blotting (WB)
- Cross-Reactivity (Details)
- No cross-reactivity with: Chromera velia
- Characteristics
- Expected / apparent Molecular Weight of the Antigene: 82 / 55-60 kDa
- Purification
- serum
- Immunogen
- N-terminal part of recombinanat PsaA protein from Chlamydomonas reinhardtii P12154
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- Application Notes
-
Recommended Dilution: 1 : 1000 with standard ECL (WB), 1 : 20 (IG).
PsaA is a hydrophobic protein and we recommend to use PVDF membrane fortransfer to assure best results - Comment
-
immunogold localization has been done in leaf material of Arabidopsis thaliana
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- For reconstitution add 200 µL of sterile water.
- Handling Advice
-
Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. - Storage
- -20 °C
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: "The response of Nannochloropsis gaditana to nitrogen starvation includes de novo biosynthesis of triacylglycerols, a decrease of chloroplast galactolipids, and reorganization of the photosynthetic apparatus." in: Eukaryotic cell, Vol. 12, Issue 5, pp. 665-76, (2013) (PubMed).
: "Composition and physiological function of the chloroplast NADH dehydrogenase-like complex in Marchantia polymorpha." in: The Plant journal : for cell and molecular biology, Vol. 72, Issue 4, pp. 683-93, (2012) (PubMed).
: "RNA silencing of hydrogenase(-like) genes and investigation of their physiological roles in the green alga Chlamydomonas reinhardtii." in: The Biochemical journal, (2010) (PubMed).
: "Dual localization of plant glutamate receptor AtGLR3.4 to plastids and plasmamembrane." in: Biochimica et biophysica acta, (2010) (PubMed).
: "Effects of Deficiency and Overdose of Group 2 Sigma Factors in Triple Inactivation Strains of Synechocystis sp. Strain PCC 6803." in: Journal of bacteriology, Vol. 193, Issue 1, pp. 265-73, (2010) (PubMed).
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: "The response of Nannochloropsis gaditana to nitrogen starvation includes de novo biosynthesis of triacylglycerols, a decrease of chloroplast galactolipids, and reorganization of the photosynthetic apparatus." in: Eukaryotic cell, Vol. 12, Issue 5, pp. 665-76, (2013) (PubMed).
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- Target
- PsaA
- Synonyms
- AAP-S antibody, F24D7.4 antibody, F24D7_4 antibody, PSA antibody, Peptidase M1 family protein antibody, AT1G63770 antibody
- Background
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AGI Code: ATCG00350
PsaA is a core protein of photosystem I. In plants and cyanobacteria, the primary step in oxygenic photosynthesis, the light induced charge separation, is driven bytwo large membrane intrinsic protein complexes, the photosystems I and II. Synonym: Photosystem I P700 chlorophyll a apoprotein A1. - Molecular Weight
- expected: 82 kDa, apparent: 55-60 kDa
- UniProt
- P12154
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