CGE1 antibody (N-Term)
The Rabbit Polyclonal anti-CGE1 antibody has been validated for IP and WB. It is suitable to detect CGE1 in samples from Chlamydomonas reinhardtii.
Quick Overview for CGE1 antibody (N-Term) (ABIN125894)
Target
CGE1
(Chloroplast GrpE Protein (CGE1))
Reactivity
Chlamydomonas reinhardtii
Host
Rabbit
Clonality
Polyclonal
Application
Immunoprecipitation (IP), Western Blotting (WB)
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Binding Specificity
- N-Term
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Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity known in the moment
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Predicted Reactivity
- Chlamydomonas reinhardtii
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Characteristics
- Expected / apparent Molecular Weight of the Antigene: 24 / 27-28 kDa (SDS PAGE)
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Purification
- serum
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Immunogen
- mature CGE1 protein of Chlamydomonas reinhardtii, UniProt: Q945T1, expressed with N-terminal hexahistidin tag in E. coli, purified with Ni-NTA
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Application Notes
- 1:10 000 with standard ECL (WB)
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 100 μL of sterile water.
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Handling Advice
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
Storage
- -20 °C
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Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- CGE1 (Chloroplast GrpE Protein (CGE1))
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Alternative Name
- CGE1
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Background
- CGE1 is a nuclear-encoded, chloroplast-targeted co-chaperone of chloroplast HSP70B. It forms dimers that interact with HSP70B in the ADP-state.
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Molecular Weight
- expected: 24 kDa, apparent: 27-28 kDa (SDS PAGE)
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UniProt
- Q945T1
Target
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