CD13 antibody

Catalog No. ABIN125974

This Rat Monoclonal antibody specifically detects CD13 in FACS and IHC (fro). It exhibits reactivity toward Mouse.

Quick Overview for CD13 antibody (ABIN125974)

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD13 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: ER-BMDM1

Product Details anti-CD13 Antibody

Specificity: SM2298P recognizes the Murine homologue of Human CD13, a 160 kDa cell surface antigen with Aminopeptidase N activity.

Purification: Affinity Chromatography on Protein G

Immunogen: Balb/c bone marrow derived Macrophages. Cells from immunised rats were fused with cells of the rat Y3-Ag1.2.3 myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Flow Cytometry: 1/10 - 1/50, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CD13

Target: CD13 (ANPEP) (Alanyl (Membrane) Aminopeptidase (ANPEP))

Alternative Name: CD13

Background: In steady state mice, the CD13 antigen is expressed primarily by dendritic cell and mature macrophage subpopulations. The level of CD13 antigen expression increases with maturation, after the monocytic stage. Bone marrow cells and peripheral blood monocytes are reported to be CD13 negative while thioglycollate-elicited macrophages express high levels of CD13. Dendritic cells, interdigitating dendritic cells, veiled cells and Langerhans cells all express CD13, which is reported to be involved in the development of immature dendritic cells from monocytes.Synonyms: ANPEP, APN, Alanyl aminopeptidase, Aminopeptidase M, Aminopeptidase N, Microsomal aminopeptidase, Myeloid plasma membrane glycoprotein CD13, PEPN, gp150

Gene ID: 16790

NCBI Accession: NP_032512

UniProt: P97449

Pathways: Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones