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KRT20 antibody

KRT20 Reactivity: Human, Pig WB, IHC (fro) Host: Mouse Monoclonal KS20-5 unconjugated
Catalog No. ABIN125990
  • Target See all KRT20 Antibodies
    KRT20 (Keratin 20 (KRT20))
    Reactivity
    • 90
    • 29
    • 27
    • 8
    • 6
    • 2
    • 2
    • 1
    • 1
    Human, Pig
    Host
    • 62
    • 29
    • 3
    • 1
    • 1
    Mouse
    Clonality
    • 52
    • 44
    Monoclonal
    Conjugate
    • 62
    • 12
    • 6
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    This KRT20 antibody is un-conjugated
    Application
    • 67
    • 46
    • 25
    • 15
    • 14
    • 12
    • 11
    • 9
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Specificity
    IT-Ks 20.5 represents an excellent marker for certain types of carcinomas such as adenocarcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. Very sensitive detection of intestestinal and gastric foveolar epithelium, urothelial umbrella cells, Merkel cells of epidermis as well as tumors originating therefrom (e.g. primary and metastatic colorectal carcinoma). Adenocarcinomas of breast, lung, endometrium and ovary (non-mucinous) as well as neuroendocrine tumors of the lung are essentially negative. Polypeptide recognized: protein IT (keratin K20, Mr 46 000, formerly also designated cytokeratin 20). Reacts with human, pig, not with rat. Reacts with HT-29, LoVo, DLD-1, SW 1116, CaCo-2, RT-4.
    Purification
    Protein A affinity chromatography
    Immunogen
    Electrophoretically purified keratin K20 from human intestinal mucosa.
    Clone
    KS20-5
    Isotype
    IgG2a
    Top Product
    Discover our top product KRT20 Primary Antibody
  • Application Notes
    Immunohistochemical on frozen sections: 1: 10, 1h at RT or over night at 2-8C. Immunohistochemical on cytological material. Immunoblotting.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore in 1 mL dist. water
    Buffer
    PBS buffer, pH 7.4 containing 0.09 % sodium azide, 0.5 % BSA
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    This product is photosensitive and should be protected from light
    Storage
    4 °C
    Storage Comment
    Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
  • Target
    KRT20 (Keratin 20 (KRT20))
    Alternative Name
    Cytokeratin 20 (KRT20 Products)
    Synonyms
    KRT20 antibody, CD20 antibody, CK-20 antibody, CK20 antibody, K20 antibody, KRT21 antibody, Krt21 antibody, 9030623C06Rik antibody, keratin 20 antibody, KRT20 antibody, Krt20 antibody
    Background
    Cytokeratin 20 is a type I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Their genes are clustered in a region of chromosome 17q12-q21. Cytokeratin 20 is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. It is also expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system and is present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack cytokeratin 20.Synonyms: CK20, Cytokeratin 20, K20, KRT20, Keratin type I cytoskeletal 20, Keratin-20, Protein IT
    Gene ID
    54474
    NCBI Accession
    NP_061883
    UniProt
    P35900
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