CD14 antibody

Catalog No. ABIN126002

The Mouse Monoclonal anti-CD14 antibody is suitable to detect CD14 in samples from Human and Primate. It has been validated for FACS, WB, IP, Func and EIA.

Quick Overview for CD14 antibody (ABIN126002)

Target: CD14

Reactivity: Human, Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func), Enzyme Immunoassay (EIA)

Clone: MEM-18

Product Details anti-CD14 Antibody

Purification: Protein A Chromatography

Immunogen: A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria

Isotype: IgG1

Application Details

Application Notes: Flow cytometry. Immunoprecipitation. Western blotting (non-denaturing conditions). ELISA: antibody MEM-18 has been tested as the detection antibody in a sandwich ELISA for analysis of human CD14 in combination with clone B-A8 (ABIN120555). Functional application: completely blocks binding of fluorescein (FITC) labeled bacterial LPS to the monocyte surface and it also blocks the binding of CD14 to the extracellular TLR2 domain.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Azide Free Phosphate Buffered Saline (PBS), pH ~7.4, 0.2 μm filter sterilized. Endotoxin level is less than 10 EU/mg of the protein, as determined by the LAL test.

Preservative: Azide free

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf Life: one year from despatch.

Expiry Date: 12 months

Target Details for CD14

Target: CD14

Alternative Name: CD14

Background: CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where serves as a multifunctional lipopolysaccharide receptor, it is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.Synonyms: Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades