Transferrin Receptor antibody

Catalog No. ABIN126028

This Mouse Monoclonal antibody specifically detects Transferrin Receptor in FACS, WB and IHC (fro). It exhibits reactivity toward Rat.

Quick Overview for Transferrin Receptor antibody (ABIN126028)

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Transferrin Receptor antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: OX-26

Product Details anti-Transferrin Receptor Antibody

Purification: Affinity chromatography on Protein G

Endotoxin Level: Low Endotoxin

Immunogen: PHA activated rat lymphocytes. Spleen cells from immunised Balb/c mice were fused with cells of the NS1 mouse myeloma cell line.

Isotype: IgG2a

Application Details

Application Notes: Western Blot: This antibody detects a band of approximately 95 kDa in ConA cell lysates under reducing conditions in Western blotting. (Mwt 195 kDa without reduction). Immunohistochemistry on frozen sections. Flow Cytometry: 1/10 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Further Comments: Endotoxin Level: less than 0.01EU/ug

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store the antibody at -20 °C. Ship at 2 - 8 °C.

Target Details for Transferrin Receptor

Target: Transferrin Receptor (TFRC) (Transferrin Receptor (p90, CD71) (TFRC))

Alternative Name: CD71 / TFRC

Background: Transferrin receptor (CD71), transferrin and ferritin have been identified as specialised proteins which control the uptake, transport and storage of free iron in tissues, thereby maintaining iron homeostasis. An imbalance in iron homeostasis within the brain has been linked with the neurodegenerative diseases, Alzheimers, Parkinsons, Huntingtons and Multiple Sclerosis.Synonyms: TfR1, Transferrin receptor protein 1, p90

Gene ID: 10116

UniProt: Q99376

Pathways: Transition Metal Ion Homeostasis