Cytokeratin 1 antibody (pan)

Catalog No. ABIN126063

Product Details anti-Cytokeratin 1 Antibody

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Binding Specificity: pan

Reactivity: Human, Mammalian

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytokeratin 1 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody reacts positively with all stratified squamous epithelia and epidermal appendages such as hairfollicles, sebaceous glands. Also positive on epithelia of the urinary tract, stomach, intestine, uterine endometrium and prostate. Identifies mesotheliomas, squamous cell carcinomas, adenocarcinomas, transitional cell carcinomas and anaplastic carcinomas.

Cross-Reactivity (Details): Species reactivity (tested):Human and most Mammals (including Mouse).

Purification: Affinity Chromatography.

Immunogen: Human skin.

Application Details

Application Notes: Immunohistochemistry on Frozen and Paraffin-Embedded Tissue (With Paraffin Sectionsproteolytic treatment with Trypsin is recommended). Working Dilution: 1/50 (optimize by serial dilution). Dilution Buffer: Dilute immediately before use with PBS or TBS. Incubation time: 1 h at room temperature. Recommended Positive Control: Squamous cell carcinoma, skin, cervix.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS or TBS, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Cytokeratin 1

Target: Cytokeratin 1 (KRT1) (Keratin 1 (KRT1))

Alternative Name: Cytokeratin (KRT1 Products)

Synonyms: CK1 antibody, EHK antibody, EHK1 antibody, EPPK antibody, K1 antibody, KRT1A antibody, NEPPK antibody, Krt-2.1 antibody, Krt2-1 antibody, Krt86 antibody, KRT1 antibody, Kb1 antibody, KER1 antibody, CK-1 antibody, Keratin-1 antibody, keratin 1 antibody, KRT1 antibody, Krt1 antibody

Background: Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Synonyms: Cytokeratin pan-reactive, pan Keratin