CTNNA1 antibody

Catalog No. ABIN126738

The Mouse Monoclonal anti-CTNNA1 antibody (Clone 15D9) (ABIN126738) specifically detects CTNNA1 in WB, IF, IP and EIA. The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for CTNNA1 antibody (ABIN126738)

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTNNA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

Clone: 15D9

Product Details anti-CTNNA1 Antibody

Specificity: This antibody specifically recognizes α-catenin in Western blot at 102 kDa.

Characteristics: Synonyms: Alpha E-catenin, Alpha Catenin, Cadherin-associated protein, NY-REN-13 antigen

Purification: Size exclusion chromatography

Components: incl. positive Control

Immunogen: Recombinant α-catenin

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.05 μg/mL. Immunoprecipitation: 1- 10 g per 10^6 vanadate-treated A431 cells. Immunocytochemistry: 0.1-1 μg/mL , mab a-CAT-15D9 may tolerate formaldehyd.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2x PBS / 0.09 % Na-azide / PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for CTNNA1

Target: CTNNA1 (Catenin (Cadherin-Associated Protein), alpha 1, 102kDa (CTNNA1))

Alternative Name: Catenin alpha-1 / CTNNA1

Background: The α-, β- and γ-catenins are cytoplasmic proteins mediating the interaction of Ca2+-dependent transmembrane adhesion molecules (cadherins) with the cytoskeletal network. The N-terminus of α-catenin directly interacts with exon 3 of β-catenin. The C-terminus of α-catenin interacts with the actin filament network.Synonyms: Alpha Catenin, Alpha E-catenin, Cadherin-associated protein, NY-REN-13 antigen

Gene ID: 1495

UniProt: P35221

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location