CK1 epsilon antibody (AA 355-380)

Catalog No. ABIN126752

The Mouse Monoclonal anti-CK1 epsilon antibody is suitable to detect CK1 epsilon in samples from Human, Mouse, Rat and Dog. It has been validated for WB and EIA.

Quick Overview for CK1 epsilon antibody (AA 355-380) (ABIN126752)

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CK1 epsilon antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 4D7

Product Details anti-CK1 epsilon Antibody

Binding Specificity: AA 355-380

Specificity: Mab CK1e-4D7 specifically recognizes the epsilon isoform of CK1.

Characteristics: Synonyms: CKI-epsilon, CKIe, CSNK1E

Purification: Purified from serum-free cell culture supernatant by subsequent thiophilic adsorption and size exclusion chromatography.

Components: incl. positive Control

Immunogen: Peptide (aa 355 - 380 of CK1e) conjugated to hemocyanin

Isotype: IgG1

Application Details

Application Notes: ELISA (0.1 μg/mL). Western blot (detects a band at 45 kD, Use at 0.5 μg/mL for HRPO/ECL detection,Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: For positive control cell lysate from serum starved A431 cells.

Restrictions: For Research Use only

Handling

Reconstitution: Reconstitute with 1 mL H2O (15 min, RT)

Buffer: 2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: For long term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution aliquoteand freeze in liquid nitrogen, reconstituted antibody can be stored frozen at -80 °C up to 1year. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months. Avoidrepeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CK1 epsilon

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Alternative Name: Casein Kinase I epsilon

Background: Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as subtrates. It can also phosphorylate a large number of proteins and participates in Wnt signaling.Synonyms: CKI-epsilon, CKIe, CSNK1E

Gene ID: 1454

UniProt: P49674

Pathways: Hedgehog Signaling, M Phase