N-Acetylglucosamine antibody

Catalog No. ABIN126802

This Mouse Monoclonal antibody specifically detects N-Acetylglucosamine in WB and EIA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for N-Acetylglucosamine antibody (ABIN126802)

Target: N-Acetylglucosamine

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 10D8

Product Details

Purification: Size exclusion chromatography

Immunogen: N-Acetylglucosamine conjugated to KLH.

Isotype: IgM

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 - 1.0 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS / 0.09 % Na-azide / PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch

Expiry Date: 12 months

Target Details

Target: N-Acetylglucosamine

Background: Many intracellular proteins contain monomeric O - linked N - acetylglucosamine residues conjugated to th hydroxyl group of serine or threonine residues. This modification can interfere with tserine and threonine phosphorylation.