ERK2 antibody (N-Term)

Catalog No. ABIN126833

The Mouse Monoclonal anti-ERK2 antibody has been validated for WB and EIA. It is suitable to detect ERK2 in samples from Human, Mouse, Rat and Dog. There is 1 publication available.

Quick Overview for ERK2 antibody (N-Term) (ABIN126833)

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERK2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 6H3

Product Details anti-ERK2 Antibody

Binding Specificity: N-Term

Specificity: This antibody specifically recognizes the N-terminus of MAP kinase 2 (ERK2).

Characteristics: Synonyms: Mitogen-activated protein kinase 1, p42-MAPK, ERT1, PRKM1, PRKM2, MAP kinase 2,MAPK2, MAPK1, Extracellular signal-regulated kinase 2

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography

Components: incl. positive Control

Immunogen: Peptide conjugated to KLHEpitope: N-terminus

Isotype: IgG1

Application Details

Application Notes: ELISA: Use at 0.05 μg/mL. Immunoblotting: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer AS00002BU-N or AS00002BU-L. Included Positive Control: Cell lysate from untreated HepG2 cells (See Protocols for moredetails).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Positive Control Provided. Cell lysate from untreated HepG2Description: Cell lysate from untreated HepG2 cells, hepatocellular carcinoma (human)Format: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H20. After complete solubilization add 200 µl2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (minigel) for HRPO/ECL detection of the target proteins. Please note: The lyophilized cell lysates contain SDS and are not recommended forapplications with native proteins such as immunoprecipitation.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS containing PEG and Sucrose, 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: For long-term storage, freeze lyophilizate upon arrival (-20 °C). Upon reconstitution, aliquote and freeze in liquid nitrogen, reconstituted antibody can bestored frozen at -80 °C up to 1 year. Avoid repeated freeze / thaw cycles. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 2-8 °C up to 3 months.

Expiry Date: 12 months

References for anti-ERK2 antibody (ABIN126833)

Samuels, Karlo, Faruzzi, Pickering, Herrup, Sweatt, Saitta, Landreth: "Deletion of ERK2 mitogen-activated protein kinase identifies its key roles in cortical neurogenesis and cognitive function." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 27, pp. 6983-95, (2008) (PubMed).

Target Details for ERK2

Target: ERK2 (MAPK1) (Mitogen-Activated Protein Kinase 1 (MAPK1))

Alternative Name: ERK2

Background: Extracellular signal/mitogen activated protein kinases (erk/MAPK) are a group of proline-directed serine/threonine kinases that are activated by dual phosphorylation of conserved threonine and tyrosine residues within a characteristic T X Y peptide motif. The mitogen-activated kinases erk1 (MAPK1) and erk2 (MAPK2) acquire full enzymatic activity upon phosphorylation of both threonine and tyrosine residues within the sequence motif T E Y.Synonyms: ERT1, Extracellular signal-regulated kinase 2, MAP kinase 2, MAPK1, MAPK2, Mitogen-activated protein kinase 1, PRKM1, PRKM2, p42-MAPK

Molecular Weight: 42 kDa

Gene ID: 5594

UniProt: P28482

Pathways: MAPK Signaling, RTK Signaling, Apoptosis, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Monocarboxylic Acid Catabolic Process, Autophagy, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, S100 Proteins