PIN1 antibody

Catalog No. ABIN126850

This Mouse Monoclonal antibody specifically detects PIN1 in WB and EIA. It exhibits reactivity toward Human, Mouse and Dog.

Quick Overview for PIN1 antibody (ABIN126850)

Target: PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))

Reactivity: Human, Mouse, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This PIN1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 8C10

Product Details anti-PIN1 Antibody

Specificity: This antibody specifically recognizes Human PIN1 in cell extracts at 16 kDa.

Characteristics: Synonyms: PIN-1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Peptidyl-prolyl cis-transisomerase Pin1

Purification: Purified from serum-free cell culture supernatant by Subsequent Thiophilic Adsorption and Size Exclusion Chromatography

Components: incl. positive Control

Immunogen: Recombinant human PIN1

Isotype: IgG1

Application Details

Application Notes: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. ELISA: 0.1 μg/mL (protein ELISA).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for PIN1

Target: PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))

Alternative Name: PIN1

Background: PIN1 is a peptidyl-prolyl-cis-trans-isomerase (PPIase) that specifically interacts with serine phosphate-proline or threonine phosphate-proline motifs. Upon binding, PIN1 isomerizes the peptide bond form cis to trans. Known substrates of PIN1 are serveral mitotic phosphoproteins (e.g. cdc25) as well as phosphorylated p53, phosphorylated β-catenin and phosphorylated tau protein. It is assumed that the isomerization of phosphoproteins regulates their biological function.Synonyms: PIN-1, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, Peptidyl-prolyl cis-trans isomerase Pin1

Gene ID: 5300

UniProt: Q13526