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PLSCR1 antibody (N-Term)

PLSCR1 Reactivity: Human WB Host: Mouse Monoclonal 1-00E-009 unconjugated
Catalog No. ABIN126886
  • Target See all PLSCR1 Antibodies
    PLSCR1 (phospholipid Scramblase 1 (PLSCR1))
    Binding Specificity
    • 7
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 27
    • 5
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 20
    • 9
    Mouse
    Clonality
    • 21
    • 8
    Monoclonal
    Conjugate
    • 22
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PLSCR1 antibody is un-conjugated
    Application
    • 18
    • 8
    • 8
    • 6
    • 5
    • 4
    • 3
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Specificity
    This antibody specifically recognizes the N-terminus of scramblase-1 at 35 kDa.
    Characteristics
    Synonyms: Phospholipid scramblase 1, PL scramblase 1, MmTRA1b, Ca(2+)-dependent phospholipidscramblase 1, Erythrocyte phospholipid scramblase
    Purification
    Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.
    Components
    incl. positive Control
    Immunogen
    Synthetic peptide conjugated to KLH.
    Clone
    1-00E-009
    Isotype
    IgG1
    Top Product
    Discover our top product PLSCR1 Primary Antibody
  • Application Notes
    Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Protocol
    Positive Control Cell LysateHepG2 untreatedSize Sufficient: 20 mini-gel lanes. Formulation: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for Immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.
    Restrictions
    For Research Use only
  • Reconstitution
    Restore with 1 mL H2O (15 min, RT).
    Buffer
    1 mLPBS containing 0.09 % Sodium Azide as preservative, PEG and Sucrose.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C/-80 °C
    Storage Comment
    Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freezing and thawing.
    Shelf life: one year from despatch.
    Expiry Date
    12 months
  • Target
    PLSCR1 (phospholipid Scramblase 1 (PLSCR1))
    Abstract
    PLSCR1 Products
    Synonyms
    mmtra1b antibody, MGC84969 antibody, PLSCR1 antibody, MGC148322 antibody, MMTRA1B antibody, MmTRA1a antibody, MmTRA1b antibody, Nor1 antibody, Tra1 antibody, Tra1a antibody, Tra1b antibody, Tras1 antibody, Tras2 antibody, PLSCR2 antibody, phospholipid scramblase 1 L homeolog antibody, phospholipid scramblase 1 antibody, plscr1.L antibody, PLS1 antibody, PVX_111580 antibody, PLSCR1 antibody, plscr1 antibody, Plscr1 antibody, LOC100712949 antibody, PLSCR2 antibody, LOC100341366 antibody, LOC611500 antibody
    Background
    Scramblase-1 is a calcium-binding protein that can be induced by interferon and growth factors (e.g. epidermal growth factor EGF). Depending on its palmitoylation state, scramblase-1 either inserts into the plasma membrane or binds DNA in the nucleus. Scramblase-1 is a substrate for src kinases.Synonyms: Ca(2+)-dependent phospholipid scramblase 1, Erythrocyte phospholipid scramblase, MmTRA1b, PL scramblase 1, Phospholipid scramblase 1
    Gene ID
    5359
    UniProt
    O15162
    Pathways
    Cellular Response to Molecule of Bacterial Origin
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