PLSCR1 antibody (N-Term)

Catalog No. ABIN126886

This Mouse Monoclonal antibody specifically detects PLSCR1 in WB. It exhibits reactivity toward Human.

Quick Overview for PLSCR1 antibody (N-Term) (ABIN126886)

Target: PLSCR1 (phospholipid Scramblase 1 (PLSCR1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PLSCR1 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 1-00E-009

Product Details anti-PLSCR1 Antibody

Binding Specificity: N-Term

Specificity: This antibody specifically recognizes the N-terminus of scramblase-1 at 35 kDa.

Characteristics: Synonyms: Phospholipid scramblase 1, PL scramblase 1, MmTRA1b, Ca(2+)-dependent phospholipidscramblase 1, Erythrocyte phospholipid scramblase

Purification: Subsequent Thiophilic Adsorption and Size Exclusion Chromatography.

Components: incl. positive Control

Immunogen: Synthetic peptide conjugated to KLH.

Isotype: IgG1

Application Details

Application Notes: Western Blot: 1 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: Positive Control Cell LysateHepG2 untreatedSize Sufficient: 20 mini-gel lanes. Formulation: Lyophilized cell lysate from serum starved HepG2 cells. Reconstitution: Restore by addition of 200 µl H2O. After complete solubilization add 200µl 2x SDS-PAGE sample buffer, mix and incubate at 90°C for 5 min. Storage: Aliquote and store frozen. Avoid repeated freeze/thaw cycles. Application: The positive control cell lysate is recommended for Immunoblot applications. 20 µl of positive control cell lysate correspond to ca. 80. 000 cells. Use 20 µl / lane (mini gel) for HRPO/ECL detection of the target proteins.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1 mL H2O (15 min, RT).

Buffer: 1 mLPBS containing 0.09 % Sodium Azide as preservative, PEG and Sucrose.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C/-80 °C

Storage Comment: Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for PLSCR1

Target: PLSCR1 (phospholipid Scramblase 1 (PLSCR1))

Background: Scramblase-1 is a calcium-binding protein that can be induced by interferon and growth factors (e.g. epidermal growth factor EGF). Depending on its palmitoylation state, scramblase-1 either inserts into the plasma membrane or binds DNA in the nucleus. Scramblase-1 is a substrate for src kinases.Synonyms: Ca(2+)-dependent phospholipid scramblase 1, Erythrocyte phospholipid scramblase, MmTRA1b, PL scramblase 1, Phospholipid scramblase 1

Gene ID: 5359

UniProt: O15162

Pathways: Cellular Response to Molecule of Bacterial Origin