Cytochrome C antibody

Catalog No. ABIN126969

The Rabbit Polyclonal anti-Cytochrome C antibody (ABIN126969) specifically detects Cytochrome C in WB and IP. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for Cytochrome C antibody (ABIN126969)

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cytochrome C antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-Cytochrome C Antibody

Sequence: surroundin g amino acid 70

Specificity: The cytochrome c antibody detects the 12.6 kDa cytochrome c.

Purification: Protein A purified

Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 70 of rat cytochrome c.

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL, Jurkat cell lysate, NIH3T3 cell lysate, and rat kidney tissue lysatecan be used as positive controls. Immunoprecipitation: 10-20 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/mL

Buffer: PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for Cytochrome C

Target: Cytochrome C (CYCS) (Cytochrome C, Somatic (CYCS))

Alternative Name: Cytochrome C

Background: Cytochrome c (m.w. 12,500) is an electron transport protein from mitochondria. It is released from mitochondria to cytoplasm during the early stages of apoptosis, prior to caspase activation, DNA fragmentation, and loss of membrane potential. The cytoplasmic cytochrome c is associated with Apaf-1 and caspase-9 to activate caspase-3 and other caspases.Synonyms: CYC, CYCS

Gene ID: 54205

NCBI Accession: NP_061820

UniProt: P99999

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity