PRKDC antibody

Catalog No. ABIN127020

The Rabbit Polyclonal anti-PRKDC antibody (ABIN127020) specifically detects PRKDC in WB. The antibody is reactive with Human, Mouse, Rat and Hamster samples.

Quick Overview for PRKDC antibody (ABIN127020)

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reactivity: Human, Mouse, Rat, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKDC antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PRKDC Antibody

Specificity: The antibody detects a >350,000 kDa and a 180,000 kDa protein (believed to be a processed form of the catalytic subunit), corresponding to the expected molecular weight of DNA-PKcs.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to residues surrounding amino acid 4088 of human DNA- PK.

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL. HeLa cell lysate can be used as a positive control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Alternative Name: DNA-PKcs / PRKDC / XRCC7

Background: DNA-dependent protein kinase (DNA-PK) is made up of 3 subunits, 2 Ku proteins and a large catalytic subunit (DNA-PKcs). DNA-PKcs falls into the phosphatidyl-inositol 3-kinase superfamily although it does have serine/threonine protein kinase activity. This subunit migrates on SDS-PAGE gels as an intact and smaller processed form. In vitro, DNA-PK phosphorylates several transcription factors and other DNA-binding proteins and is believed to play a role in DNA damage recognition, repair and transcription. There is evidence to suggest that DNA-PKcs may be a critical target for proteolysis by a caspase in apoptosis.Synonyms: DNA-dependent protein kinase catalytic subunit, DNPK1, HYRC, HYRC1, p460

Gene ID: 5591

NCBI Accession: NP_001075109

UniProt: P78527

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response