CD40 Ligand antibody

Catalog No. ABIN127037

The Rabbit Polyclonal anti-CD40 Ligand antibody is suitable to detect CD40 Ligand in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for CD40 Ligand antibody (ABIN127037)

Target: CD40 Ligand (CD40LG)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD40 Ligand antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-CD40 Ligand Antibody

Specificity: This antibody detects CD40L.

Purification: Affinity chromatography

Immunogen: Synthetic peptide surrounding amino acid 246 of human CD40 Ligand

Application Details

Application Notes: Western blot (0.5-4 μg/mL). Recommended positive control: Jurkat cell lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 30 % glycerol, 0.5 % BSA, and 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid Repeated freeze/defrost cycles.

Storage: -20 °C

Storage Comment: Store (in aliquots) at -20 °C.

Target Details for CD40 Ligand

Target: CD40 Ligand (CD40LG)

Alternative Name: CD154 / CD40L

Background: CD40 is a type I membrane protein found on the surface of B cells and primary carcinomas. It belongs to the TNF-R family. CD40 serves as the receptor for CD40 ligand (CD40L), a cytokine expressed on the cell surface of T cells. CD40 plays a critical role in B cell proliferation, antibody class switching, modulation of apoptosis in the germinal center through interaction with T cells expressing CD40L, and activation of CD4+ T cells. Ligation of CD40 with its ligand preferentially induces stress-activated protein kinases in B lymphocytes.Synonyms: CD40 ligand, CD40-L, CD40LG, GP39, T-cell antigen Gp39, TNF-related activation protein, TNFSF5, TRAP, Tumor necrosis factor ligand superfamily member 5

Gene ID: 959

NCBI Accession: NP_000065

UniProt: P29965

Pathways: NF-kappaB Signaling, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints