SMAD4 antibody

Catalog No. ABIN127142

The Rabbit Polyclonal anti-SMAD4 antibody is suitable to detect SMAD4 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for SMAD4 antibody (ABIN127142)

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD4 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-SMAD4 Antibody

Specificity: The antibody recognizes ~70 kDa Smad4.

Purification: antigen affinity purified

Immunogen: Synthetic peptide corresponding to amino acids residues around 281 of human Smad4.

Application Details

Application Notes: Western Blot: 1-4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS containing 50 % glycerol, 1 % BSA, and 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for SMAD4

Target: SMAD4 (SMAD Family Member 4 (SMAD4))

Alternative Name: SMAD4

Background: Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad), have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1, Smad5, and Smad8 are effectors of BMP2 and BMP4 function while Smad2 and Smad3 are involved in TGF-β and activin-mediated growth modulation. Smad4 has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.Synonyms: DPC4, Deletion target in pancreatic carcinoma 4, MAD homolog 4, MADH4, Mothers against DPP homolog 4, Mothers against decapentaplegic homolog 4, SMAD 4, SMAD family member 4, SMAD-4

Gene ID: 4089

NCBI Accession: NP_005350

UniProt: Q13485

Pathways: Cell Division Cycle, Chromatin Binding, Autophagy