MARCKS antibody (pSer152, pSer156)

Catalog No. ABIN127281

The Rabbit Polyclonal anti-MARCKS antibody (ABIN127281) specifically detects MARCKS in WB. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MARCKS antibody (pSer152, pSer156) (ABIN127281)

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARCKS antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MARCKS Antibody

Binding Specificity: pSer152, pSer156

Specificity: The antibody detects 80 kDa MARCKS only when phosphorylated at Ser152/156.

Purification: Affinity purified

Application Details

Application Notes: Western Blot: 1-4 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.2, containing 50 % glycerol, 1 % BSA, 0.02 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for MARCKS

Target: MARCKS (Myristoylated Alanine-Rich Protein Kinase C Substrate (MARCKS))

Alternative Name: MARCKS

Background: MARCKS (Myristoylated alanine-rich protein kinase C substrate) is a major PKC substrate that is distributed in various cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic and mitogenesis. PKC phosphorylates Ser152, 156 and 163 of MARCKS, which regulates MARCKS's calcium/calmodulin binding activity and filamentous (F)-actin cross-linking activity. In addition, phosphorylation by PKC results in translocation of MARCKS from membrane to cytoplasm.Synonyms: MACS, Myristoylated alanine-rich C-kinase substrate, PRKCSL

Gene ID: 4082

NCBI Accession: NP_002347

UniProt: P29966