BRCA2 antibody (N-Term)

Catalog No. ABIN127309

This Rabbit Polyclonal antibody specifically detects BRCA2 in WB. It exhibits reactivity toward Human and Mouse.

Quick Overview for BRCA2 antibody (N-Term) (ABIN127309)

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRCA2 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-BRCA2 Antibody

Binding Specificity: N-Term

Specificity: This antibody detects BRCA2.

Purification: Affinity Chromatography.

Immunogen: Synthetic peptide mapping to the N-terminus of Human BRCA2.

Application Details

Application Notes: Western Blot: 0.5-4 μg/mL. HeLa cell lysate can be used as a Positive Control.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: PBS pH 7.2, containing 30 % Glycerol, 0.5 % BSA and 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: -20 °C/-80 °C

Storage Comment: Store the antibody undiluted at -20 °C or for long term storage (in aliquots) at -70 °C.

Target Details for BRCA2

Target: BRCA2 (Breast Cancer 2, Early Onset (BRCA2))

Alternative Name: BRCA2 / FANCD1

Background: BRCA 1 and 2 are breast cancer susceptibility proteins that plays a role in DNA damage, repair, cell cycle progression, transcription and apoptosis, etc. The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1863 amino acids. BRCA2 has been shown to be required for localization of Rad51 to sites of DSBs (double stranded breaks) in DNA. Cells lacking BRCA1 and BRCA2 cannot repair DSBs through the Rad51-dependent process.Synonyms: Breast cancer type 2 susceptibility protein, FACD, Fanconi anemia group D1 protein

Gene ID: 675

NCBI Accession: NP_000050

UniProt: P51587

Pathways: DNA Damage Repair, M Phase, Maintenance of Protein Location