Cdc2, p34 antibody

Catalog No. ABIN129504

Product Details

Target: Cdc2, p34

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunoprecipitation (IP)

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Characteristics: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p34 cdc2 fusion protein.
Immunogentype:Recombinant

Clone: POH-1

Isotype: IgG

Application Details

Application Notes: This antibody is suitable for immunohistochemistry immunoprecipitation (as active kinase),  and immunoblotting. The antibody detects the three bands within the 34kD region corresponding to the p34 protein and its cleavage products.  HeLa cell lysate or human colon carcinoma is suggested as a positive control for immunoblotting.  LEP fibroblast cell lysate is suggested as a negative control.   Paraffin embedded tissue is reactive for immunohistochemistry using high temperature release and 0.1% saponin treatment or other permeabilization method.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References

Ye, Fincher, Tang, McNeal, Gygax, Wexler, Ryan, James, Osmani: "Proteolysis and tyrosine phosphorylation of p34cdc2/cyclin B. The role of MCM2 and initiation of DNA replication to allow tyrosine phosphorylation of p34cdc2." in: The Journal of biological chemistry, Vol. 272, Issue 52, pp. 33384-93, (1998) (PubMed).

Palmer, Gavin, Nebreda: "A link between MAP kinase and p34(cdc2)/cyclin B during oocyte maturation: p90(rsk) phosphorylates and inactivates the p34(cdc2) inhibitory kinase Myt1." in: The EMBO journal, Vol. 17, Issue 17, pp. 5037-47, (1998) (PubMed).

Target Details

Target: Cdc2, p34

Background: P34 cdc2 is a serine-threonine protein kinase of 34,000 daltons that complexes with cyclin to form maturation promoting factor (MPF).  The inactive form of the protein is phosphorylated at threonine (T) and tyrosine (Y) residues.  In humans the phosphor-ylation appears to be performed by p60src.  The active form of the protein is dephosphorylated and it functions by phosphorylating a number of proteins.  The phosphorylation activity is coupled to the entry into the M-phase of the cell.  p34 cdc2 protein must be associated with a normal cyclin protein for the M-phase to be completed normally.  Association with deletion mutants of cyclin halts the M-phase before it is completed.
Synonyms: Cell Divsion Cycle 2 Protein antibody, Cyclin Dependent Kinase 1 antibody, DKFZp686L20222 antibody, MGC111195 antibody, p34 antibody, p34 Cdk1 antibody, p34 protein kinase antibody

Gene ID: 983

UniProt: P06493