CD97 antibody (AA 1-512)

Catalog No. ABIN129531

Supplier Product No.: 200-401-908

Product Details anti-CD97 Antibody

Target: CD97

Binding Specificity: AA 1-512

Reactivity: Human, Chimpanzee

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD97 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Supplier Product No.: 200-401-908

Supplier: Rockland

Purpose: CD97 Antibody

Cross-Reactivity (Details): This Protein A purified antibody is directed against the mouse CD97 protein.

Characteristics: Synonyms: rabbit anti-CD97 Antibody, CD97 antigen antibody, CD97 Molecule antibody, Leukocyte antigen CD97 antibody, CD97 antigen subunit alpha, CD97 antigen subunit beta

Purification: The product was protein A purified from monospecific antiserum.

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-512 (extracellular domain) of mouse CD97 protein.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This Protein A purified antibody has been tested for use in immunofluorescence microscopy and western blot.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 81 kDa in size corresponding to isoform 1 of CD97 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:10,000 - 1:50,000

IF Microscopy Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 5.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for CD97

Target: CD97

Alternative Name: Cd97 (CD97 Products)

Background: Background: This antibody is designed, produced, and validated as part of a collaboration with the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. CD97 is a membrane antigen that is either constitutively expressed or induced by activation on cells of the immune system including colon, thyroid, stomach, and esophagus. One high affinity ligand that has been identified for CD97 is the integrin alpha 5 beta 1. CD97 engagement of endothelial alpha5 beta 1 results in increased angiogenesis. Signals generated through CD97 are thought to result in Rho activation and the regulation of motility. Anti-CD97 is ideal for researchers interested in cancer, angiogenesis, and inflammation.

Gene ID: 26364, 251823835

UniProt: Q9Z0M6