CD97 antibody (CD97)

Details for Product anti-CD97 Antibody No. ABIN94252
Antigen
Reactivity
Human
152
41
22
4
2
Host
Mouse
104
56
8
4
Clonality (Clone)
Monoclonal ()
Conjugate
This CD97 antibody is un-conjugated
16
12
8
7
5
4
3
3
3
3
3
3
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
91
58
50
48
23
16
13
7
4
3
2
1
1
1
1
1
1
Options
Immunogen PHA-activated peripheral blood cells
Clone MEM-180
Isotype IgG1
Specificity The antibody MEM-180 recognizes a unique epitope on CD97, a 75-85 kDa surface glycoprotein of G-protein-coupled receptor family, expressed on activated B and T lymphocytes, monocytes/macrophages, dendritic cells and granulocytes.
HLDA VI, WS Code BP 415
HLDA VI, WS Code NL N-L023
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD97 (CD97 Antibody Abstract)
Background CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. CD97 is produced in alternatively spliced forms and its cellular ligand is CD55 (DAF), which protects various cell types from complement-mediated damage. Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. CD97 is involved in T cell regulation and peripheral granulocyte homeostasis.
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Product cited in: Wobus, Vogel, Schmücking, Hamann, Aust: "N-glycosylation of CD97 within the EGF domains is crucial for epitope accessibility in normal and malignant cells as well as CD55 ligand binding." in: International journal of cancer. Journal international du cancer, Vol. 112, Issue 5, pp. 815-22, 2004 (PubMed).