CD97 antibody (CD97) (FITC)

Details for Product anti-CD97 Antibody No. ABIN94253
Antigen
Reactivity
Human
152
41
22
4
2
Host
Mouse
104
56
8
4
Clonality (Clone)
Monoclonal ()
Conjugate
This CD97 antibody is conjugated to FITC
15
12
8
7
5
4
3
3
3
3
3
3
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP)
91
58
50
48
23
16
13
7
4
3
2
1
1
1
1
1
1
Options
Immunogen PHA-activated peripheral blood cells
Clone MEM-180
Isotype IgG1
Specificity The antibody MEM-180 recognizes a unique epitope on CD97, a 75-85 kDa surface glycoprotein of G-protein-coupled receptor family, expressed on activated B and T lymphocytes, monocytes/macrophages, dendritic cells and granulocytes.
HLDA VI, WS Code BP 415
HLDA VI, WS Code NL N-L023
Cross-Reactivity (Details) Human
Antigen
Alternative Name CD97 (CD97 Antibody Abstract)
Background CD97 is a G-protein-coupled seven-span transmembrane adhesive receptor that is constitutively expressed on granulocytes and monocytes and rapidly upregulated on T and B cells upon activation. CD97 is produced in alternatively spliced forms and its cellular ligand is CD55 (DAF), which protects various cell types from complement-mediated damage. Interaction of CD97 on leukocytes and CD55 on vessel cells probably facilitate leukocyte activation and migration into the tissues, similarly, CD97 seems to play a role in tumour migration and invasiveness. CD97 is involved in T cell regulation and peripheral granulocyte homeostasis.
Application Notes The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μ,l reagent / 100 μ,l of whole blood or 106 cells in a suspension.The content of a vial (2 ml) is sufficient for 100 tests.
Comment

The purified antibody is conjugated with Fluorescein isothiocyanate (FITC) under optimum conditions. The reagent is free of unconjugated FITC and adjusted for direct use. No reconstitution is necessary.

Restrictions For Research Use only
Reconstitution No reconstitution is necessary.
Buffer The reagent is provided in stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Avoid prolonged exposure to light.
Storage 4 °C
Storage Comment Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD97 (CD97) antibody (FITC) (ABIN94253) Surface staining of human peripheral blood with anti-CD97 (MEM-180) FITC.
Image no. 2 for anti-CD97 (CD97) antibody (FITC) (ABIN94253) Surface staining of human peripheral blood with anti-CD97 (MEM-180) FITC.
Product cited in: Wobus, Vogel, Schmücking, Hamann, Aust: "N-glycosylation of CD97 within the EGF domains is crucial for epitope accessibility in normal and malignant cells as well as CD55 ligand binding." in: International journal of cancer. Journal international du cancer, Vol. 112, Issue 5, pp. 815-22, 2004 (PubMed).