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GFP antibody (Green Fluorescent Protein) (AA 246) (FITC) Primary Antibody

GFP Reactivity: Aequorea victoria ELISA, WB Host: Mouse Monoclonal 9F9-F9 FITC
Pubmed (1)
Catalog No. ABIN129565
$1,060.50
Plus shipping costs $45.00
1 mg
local_shipping Shipping to: United States
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  • Target
    GFP
    Binding Specificity
    AA 246
    • 15
    • 7
    • 7
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Reactivity
    Aequorea victoria
    • 215
    • 7
    • 6
    • 6
    • 6
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    Host
    Mouse
    Clonality
    Monoclonal
    Conjugate
    This GFP antibody is conjugated to FITC
    • 136
    • 15
    • 12
    • 10
    • 7
    • 6
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Application
    ELISA, Western Blotting (WB)
    • 175
    • 112
    • 56
    • 50
    • 37
    • 34
    • 32
    • 22
    • 14
    • 13
    • 12
    • 8
    • 6
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    Cross-Reactivity
    recombinant GFP (rGFP), enhanced GFP (eGFP)
    Immunogen
    The immunogen is a GST- Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence derived from the jellyfish Aequorea victoria.
    Immunogentype:Recombinant
    Clone
    9F9-F9
    Isotype
    IgG
  • Application Notes
    Monoclonal anti-GFP is designed to detect enhanced GFP and GFP containing recombinant proteins. This antibody can be used to detect GFP by ELISA (sandwich or capture) for the direct binding of antigen. Biotin conjugated monoclonal anti-GFP is well suited to titrate GFP in a sandwich ELISA in combination with polyclonal anti-GFP (600-101-215) as the capture antibody. Only use the monoclonal form for the detection of enhanced or recombinant GFP. Polyclonal anti-GFP detects all variants of GFP tested to date. The biotin conjugated detection antibody is typically used with streptavidin conjugated HRP or other streptavidin conjugates. The use of polyclonal anti-GFP results in significant amplification of signal when fluorochrome conjugated polyclonal anti-GFP is used relative to the fluorescence of GFP alone. For immunoblotting use either alkaline phosphatase or peroxidase conjugated anti-GFP to detect GFP or GFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Restore with deionized water (or equivalent)
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
  • Marks, Nowyhed, Choi, Poholek, Odegard, Flavell, Craft: "Thymic self-reactivity selects natural interleukin 17-producing T cells that can regulate peripheral inflammation." in: Nature immunology, Vol. 10, Issue 10, pp. 1125-32, 2009 (PubMed).

  • Target
    GFP
    Alternative Name
    GFP ()
    Synonyms
    green fluorescent protein, gfp
    Background
    Green fluorescent protein is a 27 kDa protein produced from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509nm) when excited by blue light. GFP is an important tool in cell biology research. GFP is widely used enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining.
    Synonyms: FITC, GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish.
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