PRKDC antibody (pThr2609)

Catalog No. ABIN129603

Supplier Product No.: 600-401-494

This anti-PRKDC antibody is a Rabbit Polyclonal antibody detecting PRKDC in ELISA, WB and IP. Suitable for Human. This Primary Antibody has been cited in 5+ publications.

Quick Overview for PRKDC antibody (pThr2609) (ABIN129603)

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKDC antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-PRKDC Antibody

Binding Specificity: pThr2609

Supplier Product No.: 600-401-494

Supplier: Rockland

Purpose: DNA PKcs phosphoT2609 Antibody

Cross-Reactivity (Details): DNA PKcs pT2609 antibody is directed against the phosphorylated form of human DNA PKcs at the pT2609 residue.

Characteristics: Synonyms: rabbit anti-DNA PKcs pT2609 Antibody, DNA dependent protein kinase catalytic subunit antibody, Protein Kinase DNA-Activated Catalytic Polypeptide, DNA PK catalytic subunit antibody, DNA-PK antibody, DNPK-1 antibody, DNAPK catalytic subunit antibody, DNPK 1 antibody, DNPK1 antibody, HYRC1 antibody, HYRC, P460, Hyper-Radiosensitivity Of Murine Scid Mutation Complementing 1, IMD26, XRCC7, P350

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: Rabbit Anti-DNA PKcs pT2609 affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding Thr 2609 of human DNA PKcs.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-DNA PKcs pT2609 antibody has been tested for use in ELISA, western blot, and IP.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 460 kDa in size corresponding to DNA PKcs by western blotting in the appropriate cell lysate or extract.   Alternate splice variants have been described for this protein.  Best western blotting results are seen when IP is performed prior to detection.  This antibody detects an inducible signal at the correct height after DNA damage.  However, the antibody may also detect an IR-inducible signal in cells lacking DNA PKcs (MO59J cells) at the same size as DNA PKcs if western blotting is performed directly.  We believe that this additional band is 53BP1 that runs at the same size as DNA PKcs and is also phosphorylated in an IR-dependent manner. Similar results were seen with another phospho DNA PKcs antibody indicating that this result is general to antibodies to this phospho site, rather than specific to this antibody.

Western Blot Dilution: 1:500 - 1:2,000

Immunoprecipitation Dilution: User Optimized

ELISA Dilution: 1:300,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.93 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-PRKDC antibody (ABIN129603)

Yoshikawa, Kashino, Ono, Watanabe: "Phosphorylated H2AX foci in tumor cells have no correlation with their radiation sensitivities." in: Journal of radiation research, Vol. 50, Issue 2, pp. 151-60, (2009) (PubMed).

Wanner, Mayer, Kehlbach, Rodemann, Dittmann: "Activation of protein kinase Cepsilon stimulates DNA-repair via epidermal growth factor receptor nuclear accumulation." in: Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, Vol. 86, Issue 3, pp. 383-90, (2008) (PubMed).

Dittmann, Mayer, Kehlbach, Rodemann: "The radioprotector Bowman-Birk proteinase inhibitor stimulates DNA repair via epidermal growth factor receptor phosphorylation and nuclear transport." in: Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, Vol. 86, Issue 3, pp. 375-82, (2008) (PubMed).

Dittmann, Mayer, Wanner, Kehlbach, Rodemann: "The radioprotector O-phospho-tyrosine stimulates DNA-repair via epidermal growth factor receptor- and DNA-dependent kinase phosphorylation." in: Radiotherapy and oncology : journal of the European Society for Therapeutic Radiology and Oncology, Vol. 84, Issue 3, pp. 328-34, (2007) (PubMed).

Dittmann, Mayer, Ohneseit, Raju, Andratschke, Milas, Rodemann: "Celecoxib induced tumor cell radiosensitization by inhibiting radiation induced nuclear EGFR transport and DNA-repair: a COX-2 independent mechanism." in: International journal of radiation oncology, biology, physics, Vol. 70, Issue 1, pp. 203-12, (2007) (PubMed).

Target Details for PRKDC

Target: PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide (PRKDC))

Alternative Name: PRKDC

Background: Background: DNA dependent Protein Kinase (also called DNAPK, DNPK1, HYRC1, Protein Kinase DNA Activated Catalytic Polypeptide, XRCC7 and P460) consists of the 460 kDa DNA PKcs and a heterodimeric regulatory complex comprised of p70 Ku and p80 Ku (Ku autoantigen). DNA PKcs is a nuclear protein serine/threonine kinase present in a wide variety of eukaryotic species. DNA PKcs phosphorylates transcription factors, Sp1, Oct-1, p53 and SV40 large T antigen. DNA PKcs is involved in repairing double stranded DNA breaks. At the onset of apoptosis, DNA PKcs is rapidly inactivated by cleavage of the catalytic subunit into smaller polypeptides. Proteolysis of DNA PKcs is inhibited by the cysteine protease inhibitors iodoacetamide and N-ethylmaleimide.  Alternative splicing can occur for this protein to produce at least two isoforms. Rabbit Anti-DNA PKcs pT2609 Antibody is useful for researchers interested in DNA damage.

Gene ID: 5591, 13654237

UniProt: P78527

Pathways: DNA Damage Repair, Production of Molecular Mediator of Immune Response