APC1 antibody (Ser355)

Catalog No. ABIN129637

Supplier Product No.: 600-401-857

This Rabbit Polyclonal antibody specifically detects APC1 in ELISA, WB and IP. It exhibits reactivity toward Human.

Quick Overview for APC1 antibody (Ser355) (ABIN129637)

Target: APC1 (ANAPC1) (Anaphase Promoting Complex Subunit 1 (ANAPC1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APC1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-APC1 Antibody

Binding Specificity: Ser355

Supplier Product No.: 600-401-857

Supplier: Rockland

Purpose: APC1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human APC1at the S355 residue.

Characteristics: Synonyms: rabbit anti-APC1 Antibody, APC-1, APC 1, rabbit anti-APC1 S355 Antibody, Anaphase-promoting complex subunit 1, cyclosome subunit 1, Testis-specific gene 24 protein, Mitotic checkpoint regulator

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen:

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 350-375 of Human Apc 1. Swiss Prot accession number Q9H1A4.

Immunogen Type: Conjugated Peptide

Isotype: IgG

Application Details

Application Notes:

Application Note: This affinity purified antibody has been tested for use in ELISA against the immunizing peptide.  Reactivity in other immunoassays is unknown.

Western Blot Dilution: 1:500 - 1:5,000

Immunoprecipitation Dilution: 1:100

ELISA Dilution: 1:5,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.2 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for APC1

Target: APC1 (ANAPC1) (Anaphase Promoting Complex Subunit 1 (ANAPC1))

Alternative Name: ANAPC1, TSG24

Background: Background: APC1 (also known as Anaphase promoting complex subunit 1, Cyclosome subunit 1, Protein Tsg24, Mitotic checkpoint regulator and ANAPC1) is 1 of at least 11 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.

Gene ID: 64682, 12056971

UniProt: Q9H1A4