PRKAR2A antibody

Catalog No. ABIN1302261

The Mouse Monoclonal anti-PRKAR2A antibody is suitable to detect PRKAR2A in samples from Human. It has been validated for WB and ICC.

Quick Overview for PRKAR2A antibody (ABIN1302261)

Target: PRKAR2A (Protein Kinase, CAMP-Dependent, Regulatory, Type II, alpha (PRKAR2A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PRKAR2A antibody is un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Clone: Hs-36

Product Details anti-PRKAR2A Antibody

Purpose: Anti-PRKAR2A Purified

Cross-Reactivity (Details): Human

Characteristics: The antibody Hs-36 reacts with PRKAR2A (protein kinase A regulatory type II alpha subunit), an intra-acrosomal protein.

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Purity: > 95 % (by SDS-PAGE)

Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.

Isotype: IgM

Application Details

Application Notes: Immunocytochemistry: Recommended dilution: 10 μg/mL, membrane permeabilization (acetone) is essential.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for PRKAR2A

Target: PRKAR2A (Protein Kinase, CAMP-Dependent, Regulatory, Type II, alpha (PRKAR2A))

Alternative Name: PRKAR2A

Background: Protein kinase cAMP-dependent type II regulatory s,PRKAR2A (proteinkinase A regulatory type II alpha subunit), also known as PKR2, or PRKAR2, is a component of cAMP-dependent protein kinase complex. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The PRKAR2A subunit has been shown to regulate protein transport from endosomes to the Golgi apparatus and further to the endoplasmic reticulum (ER). In sperm, this antigen can be used as an intra-acrosomal marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,PKAR2A, PKR2, PKAR2, proteinkinase A regulatory 2

Gene ID: 5576

UniProt: P13861

Pathways: Hedgehog Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, SARS-CoV-2 Protein Interactome, The Global Phosphorylation Landscape of SARS-CoV-2 Infection