Lambda-IgLC antibody (APC)

Catalog No. ABIN1302655

This Mouse Monoclonal antibody specifically detects Lambda-IgLC in FACS. It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for Lambda-IgLC antibody (APC) (ABIN1302655)

Target: Lambda-IgLC (lambda Light Chain (Lambda-IgLC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Lambda-IgLC antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: 1-155-2

Product Details anti-Lambda-IgLC Antibody (APC)

Purpose: Anti-Hu Ig Lambda Light Chain APC

Specificity: The mouse monoclonal antibody 1-155-2 recognizes lambda light chains (22.5 kDa) of human immunoglobulin.

Cross-Reactivity (Details): Human

Purification: Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human IgA1 lambda myeloma protein

Isotype: IgG1 kappa

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Extracellular and intracellular staining.

Restrictions: For Research Use only

Handling

Reconstitution: No reconstitution is necessary.

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.
Avoid prolonged exposure to light.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

References for anti-Lambda-IgLC antibody (APC) (ABIN1302655)

Tierens, Holte, Warsame, Ikonomou, Wang, Chan, Delabie: "Low levels of monoclonal small B cells in the bone marrow of patients with diffuse large B-cell lymphoma of activated B-cell type but not of germinal center B-cell type." in: Haematologica, Vol. 95, Issue 8, pp. 1334-41, (2010) (PubMed).

Bahler, Pindzola, Swerdlow: "Splenic marginal zone lymphomas appear to originate from different B cell types." in: The American journal of pathology, Vol. 161, Issue 1, pp. 81-8, (2002) (PubMed).

Kubagawa, Gathings, Levitt, Kearney, Cooper: "Immunoglobulin isotype expression of normal pre-B cells as determined by immunofluorescence." in: Journal of clinical immunology, Vol. 2, Issue 4, pp. 264-9, (1983) (PubMed).

Target Details for Lambda-IgLC

Target: Lambda-IgLC (lambda Light Chain (Lambda-IgLC))

Alternative Name: Ig Lambda Light Chain

Background: Immunoglobulin classes share the same basic four polypeptide chain structure of two heavy chains (five heavy chains types) and two light chains (kappa, lambda, both having a molecular weight of 22.5 kDa). Kappa and lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of kappa to lambda is 70:30.,Immunoglobulin lambda, Igl

Molecular Weight: 22.5 kDa